卷柏抗肿瘤转移的活性成分  

Anti-tumor metastatic constituents from Selaginella tamariscina(Beauv.) Spring

作　　者：齐妍[1] 

机构地区：[1]天津医科大学第二医院,天津300211

出　　处：《中成药》2014年第8期1682-1687,共6页Chinese Traditional Patent Medicine

摘　　要：目的研究卷柏抗肿瘤转移的活性成分。方法卷柏全草采用70%乙醇提取、系统溶剂萃取,综合运用硅胶柱色谱、凝胶柱色谱、制备HPLC色谱等分离色谱法分离纯化卷柏中的化学成分;采用NMR和质谱等有机波谱学方法鉴定化合物的结构;通过伤口愈合实验来评价卷柏化学成分抗肿瘤转移的活性。结果从卷柏中分离鉴定了7个化合物,分别为阿罗托双黄酮(1),扁柏双黄酮(2),罗波斯塔黄酮(3),异柳杉双黄酮(4),卷柏酸(5),麦芽碱-O-α-L吡喃鼠李糖苷(6),麦芽碱-O-[(6″-O-反式肉桂酰基)-4'-O-β-D-吡喃葡萄糖基-α-L-吡喃鼠李糖苷](7)。此外抗肿瘤转移活性筛选结果表明,化合物6和7均能剂量依赖性提高抗肿瘤转移活性。结论化合物5～7为首次从该植物中分离得到,化合物6和7与阳性对照药LY294002相比能够更强地抑制乳腺癌MDA-MB-231细胞的迁移的抗肿瘤转移活性。AIM To study the chemical constituents and their anti-tumor metastatic activity from the whole plant of Selaginella tamariscina （ Beauv. ） Spring. METHODS Extracted with 75% ethyl alcohol, the constit- uents were isolated through chromatography of silica gel, Toyopearl HW-40 and p-HPLC. Their structures were i- dentified on the basis of the physical properties and spectral analysis by UV,1H-NMR,13 C-NMR, ESI-MS. Wound healing assays were used to assay the anti-tumor metastatic activity of constituents from S. tamariscina. RESULTS Seven compounds were isolated from S. tamariscina and identified as amentoflavone （ 1 ） , hinokifla- vone （ 2 ）, robustaflavone （ 3 ）, isocryptomerin （ 4 ）, selaginellic acid （ 5 ）, horde-nine-O-α-L-rhamnopyranoside （6）, and hordenine-O- [（6＂-O-trans-cinnamoyl） -4＇-O-β-D-glucopyranosyl-ot-L-rhamnopyranosi-de ] （7）. In addition, the screening results of anti-tumor metastatic activity exhibited that compound 6 and 7 could inhibit tumor metastasis in dose-dependent manner. CONCLUSION Compounds 5 -7 are isolated from this plant for the first time and compound 6 and 7 exhibit the excellent anti-tumor metastatic activity on mammary cancer MDA- MB-231 cell motility.

关 键 词：卷柏 麦芽碱-O-α-L吡喃鼠李糖苷 麦芽碱-O-[(6″-O-反式肉桂酰基)-4'-O-β-D-吡喃葡萄糖基-α-L-吡喃鼠李糖苷] 抗肿瘤转移活性 

分 类 号：R284.1[医药卫生—中药学]