泽泻总三萜提取纯化工艺的实验室试验及中试验证  

Laboratory Scale Test and Pilot Scale Validation of Extraction and Purifi cation of Total Triterpenes from Alisma orientalis(Sam.) Juzep.

作　　者：罗奋熔 丘建芳[1] 许文[1] 黄鸣清[1] 吴水生[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国医药工业杂志》2014年第8期729-733,749,共6页Chinese Journal of Pharmaceuticals

基　　金：国家自然科学基金资助课题(U1205022);国家"十二五"科技支撑项目(2011BAI01B06);福建省科技厅重大专项(2009YZ0001-1-5);福建省卫生厅课题(2013-2-55)

摘　　要：以泽泻提取物中总三萜(1)和23-乙酰泽泻醇B(2)含量为评价指标;优化泽泻前处理、提取和纯化方法。所得最优工艺为:取过10目筛的泽泻粉末,用10倍量85%乙醇回流提取2次,每次1 h。所得提取液浓缩至每1 ml含0.5 g生药的溶液,取100 ml以2 BV/h(60 ml/h)的速度上D101大孔吸附树脂柱,上样完成后用水4 BV洗脱,再用6 BV 40%乙醇以4 BV/h的速度除杂,8 BV 80%乙醇以2 BV/h的流速富集1。实验室小试至50 kg规模的中试试验表明,该工艺稳定可行,所得提取物中1含量大于50%,得率约77%,2的含量约为8%。The pretreatment, extraction and purification methods of Alisma orientalis （Sam.） Juzep. were optimized with contents of total triterpenes （1） and alisol B 23-acetate （2） as indexes. The optimized process was as follows： the crude herbs about 10 mesh were extracted under reflux by 10 times of 85 % ethanol twice, 1 h pre time. The extract containing crude drug 0.5 g/ml with the volume of 100 ml was loaded on the D101 macroporous resin column with the sample flow rate of 2 BV/h （60 ml/h）. Then the column was washed by 4 BV of water, 6 BV of 40 % ethanol with the rate of 4 BV/h for removing impurities and 8 BV of 80 % ethanol with 2 BV/h for purifying 1. Both the laboratory scale test and pilot scale test of 50 kg showed that the optimal process was stable. Contents of 1 and 2 in the extract obtained by this process were above 50 % and 8 %, the yield of 1 was about 77 %.

关 键 词：泽泻 总三萜 23-乙酰泽泻醇B 中试 提取:纯化 大孔树脂 

分 类 号：R284.2[医药卫生—中药学]