注射用复方三维B(Ⅱ)中三组分的HPLC法测定  

Determination of Three Constituents in Compound Trivitamin B for Injection(Ⅱ) by HPLC

作　　者：苏彦斌[1] 张凤荣 苏彦文[1] 许永超[1] 王旭峰[1] 

机构地区：[1]吉林化工学院,吉林吉林132022 [2]吉林省食品药品检验所,吉林长春130033

出　　处：《中国医药工业杂志》2014年第8期767-770,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱法测定注射用复方三维B（Ⅱ）中的三组分.采用Agela C18色谱柱,以磷酸盐缓冲液（0.05 mol/L磷酸二氢钾溶液1 000 ml,用l mol/L氢氧化钾溶液调节pH值至6.3,A）∶甲醇（B）（86∶1 4）为流动相,测定硝酸硫胺和盐酸吡哆辛,检测波长267 nm;以A∶B（75∶25）为流动相,测定维生素B12,检测波长361 nm.硝酸硫胺、盐酸吡哆辛和维生素B12分别在2.0～10.1、30.5～152.7和1.2～6.2 μg/ml浓度范围内线性关系良好,平均回收率分别为99.63％、99.39％和100.60％,RSD分别为0.31％、0.67％和0.58％.An HPLC method was established for the determination of three constituents in compound trivitamin B for injection （ Ⅱ ）. An Agela C18 column was used with the mobile phase of phosphate buffer （0.05 mol/L potassium dihydrogen phosphate solution 1 000 ml, adjusted to pH 6.3 by 1 mol/L potassium hydroxide solution, A） ： methanol （B） （86 ： 14） at the detection wavelength of 267 nm for the determination of thiamine nitrate and pyridoxine hydrochloride, and A ： B （75 ： 25） at the detection wavelength of 361 nm for the determination of vitamin B12. The calibration curves of thiamine nitrate, pyridoxine hydrochloride and vitamin B12 were linear in the ranges of 2.0 - 10.1, 30.5- 152.7 and 1.2 - 6.2 ktg/ml, respectively. The average recoveries were 99.63 %, 99.39% and 100.60%, with RSDs of 0.31%, 0.67% and 0.58%, respectively.

关 键 词：注射用复方三维B(Ⅱ) 硝酸硫胺 盐酸吡哆辛 维生素B12 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]