蛇伤胶囊对竹叶青蛇伤兔血小板功能的影响及其作用机制研究  

Effect of Sheshang capsule on platelet function of rabbits bitten by Trimeresurus stejnegeri and its mechanism

作　　者：文丹[1] 何卫东[1] 王缓缓[1] 陈腾飞[1] 王华新[1] 吴晖[1] 吴天生[1] 邵丹[1] 李明[1] 

机构地区：[1]福建中医药大学附属人民医院急诊科,福州350004

出　　处：《中华危重病急救医学》2014年第8期585-588,共4页Chinese Critical Care Medicine

基　　金：福建省自然科学基金（2012J01379）;国家自然科学基金（81302978,81241118）;陈可冀中西医结合发展基金资助项目（CKJ2011029）

摘　　要：目的 探讨蛇伤胶囊对竹叶青蛇伤兔凝血功能的影响及机制.方法 通过0.75、1.50、2.25、3.00 mL/kg竹叶青蛇毒液染毒72 h兔实验,观察竹叶青蛇伤兔血小板聚集率的变化.将50只新西兰大白兔按照随机数字表法分为5组,每组10只.采用皮下注射0.75 mL/kg竹叶青蛇毒液制备兔中毒模型;假伤组注射0.75 mL/kg生理盐水;6h后蛇伤胶囊低、中、高剂量组分别灌胃5、10、15 mL· kg-1·d-1蛇伤胶囊药液,假伤组和模型组灌胃10 mL·kg-1·d-1生理盐水.连续灌胃1周后取血,测定血小板聚集率、血小板计数（PLT）、平均血小板体积（MPV）、血小板压积（PCT）、血小板分布宽度（PDW）和血清环磷酸腺苷（cAMP）、蛋白激酶A（PKA）含量.结果 ①随着蛇毒浓度增加,1、3、5 min及最大血小板聚集率均呈逐渐下降趋势.②模型组5 min血小板聚集率和最大血小板聚集率均较假伤组明显下降[35.5（24.2,42.5）％比43.0（38.2,58.5）％,39.5（29.0,45.0）％比46.5（39.2,60.2）％,均P＜ 0.05];与模型组比较,蛇伤胶囊中剂量组5 min血小板聚集率和最大血小板聚集率明显升高[44.0（39.8,45.0）％比35.5（24.2,42.5）％,45.5（43.5,46.2）％比39.5（29.0,45.0）％,均P＜0.05],其余各组间比较差异均无统计学意义.与假伤组比较,模型组PLT明显减少（×109/L：410.3±155.3比724.5±220.7,P＜0.01）,MPV、PCT明显缩小[MPV（fl）：5.11±1.09比6.34±1.16,P＜0.01;PCT：21.9（18.6,26.8）％比34.8（24.8,45.4）％,P＜ 0.05].与模型组比较,蛇伤胶囊低、中、高剂量组PLT、PCT均明显增加[PLT（×109/L）：702.4±166.3、648.5±160.2、789.3±86.2比410.3±155.3,PCT：38.8（35.7,42.9）％、36.0（29.8,44.4）％、43.1（40.5,48.8）％比21.9（18.6,26.8）％,均P＜0.01];蛇伤胶囊中剂量组MPV明显增大（fl：6.26±1.05比5.11±1.09,P＜0.01）.各组PDW比较差异均无统计学意义（P＞0.05）.与假伤组比较,模型组血清cAMP（nmol/L：47.57±12.7Objective To investigate the effect of the Sheshang capsule on coagulation of the rabbits bitten by Trimeresurus stejnegeri and its mechanism.Methods The changes in platelet aggregation rate was observed after the establishment of rabbits model by subcutaneously injection with 0.75,1.50,2.25,and 3.00 mL/kg of Trimeresurus stejnegeri venom for 72 hours.Fifty New Zealand white rabbits were randomly divided into five groups,10 rabbits in each group.Rabbit model was reproduced by subcutaneously injection with 0.75 mL/kg of Trimeresurus stejnegeri venom.The rabbits in sham group were injected with 0.75 mL/kg normal saline（NS）.The rabbits were gavaged with 5,10,15 mL· kg-1·d-1 of the Sheshang concoction to the low,intermediate and high dose groups respectively after 6 hours,and 10 mL· kg-1 ·d-1 NS was fed in the sham group and model group.The platelet aggregation rate,platelet count （PLT）,mean platelet volume （MPV）,plateletcrit （PCT）,platelet distribution width （PDW）,cyclic adenosine monophosphate （cAMP） and protein kinase A （PKA） were determined after 1 week.Results ① With the increase in the concentration of Trimeresurus stejnegeri venom,1-min,3-min,5-min and maximum platelet aggregation rates showed a gradual declining trends.② Compared with the sham group,5-min and maximum platelet aggregation rate in the model group were significantly decreased [35.5 （24.2,42.5）% vs.43.0 （38.2,58.5）%,39.5 （29.0,45.0）% vs.46.5 （39.2,60.2）%,both P〈0.05].Compared with the model group,5-min and maximum platelet aggregation rate in the intermediate dose group were significantly increased [44.0 （39.8,45.0）% vs.35.5 （24.2,42.5）%,45.5 （43.5,46.2）% vs.39.5 （29.0,45.0）%,both P〈0.05].There was no significant difference in platelet aggregation rate among the other groups.Compared with the sham group,PLT count in model group was obviously reduced （× 109/L：410.3 ± 155.3 vs.724.5 ± 220.7,P〈0.01）,so as MPV and PCT done [MPV （fl）：5.11 ± 1.09 vs.6.34 ± 1.16,P

关 键 词：蛇伤胶囊 竹叶青蛇 凝血障碍 血小板 血小板聚集 

分 类 号：R285.5[医药卫生—中药学]