Maculosin对肺成纤维细胞纤维化相关基因表达的影响  

Effect of Maculosin on fibrotic gene expression in lung fibroblasts

作　　者：周兴春[1] 李英新[1] 洪文[1] 王颖[1] 叶波平[1] 

机构地区：[1]中国药科大学生命科学与技术学院海洋药用生物资源实验室,南京210009

出　　处：《中国药科大学学报》2014年第4期491-495,共5页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81072571);国家"重大新药创制"科技重大专项基金资助项目(No.2012ZX09103301-018)~~

摘　　要：Maculosin是从红海榄根际土壤来源的曲霉属真菌菌株CGD12发酵液中分离得到的一种二酮哌嗪类化合物,由L-脯氨酸和L-酪氨酸脱水缩合而成。在本研究中,采用乳酸脱氢酶(LDH)法检测了Maculosin对肺成纤维细胞株的细胞毒性,通过实时荧光定量RT-PCR(qRT-PCR)评价了其对纤维化相关基因表达的影响。结果表明:Maculosin能明显抑制由外源转化生长因子-β2诱导的肺成纤维细胞株的活化,对细胞外基质(ECM)成分的合成有一定的抑制作用。进一步的分析结果显示:Maculosin的抗纤维化作用可能与其抑制炎症相关因子的表达和促进ECM成分的降解过程有关。Maculosin is a kind of cyclic dipeptides resulting from double condensations between L-proline and L- tyrosine. It was purified from the fermentation broth of strain CGD12, an Aspergillus fungi isolated from Rhizophora stylosa rhizosphere soil in the previous study. In the present study, lactate dehydrogenase （LDH） assay was applied to detect the cytotoxicity of Maculosin in lung fibroblasts. Real-time quantitative RT-PCR was used to assess its effects on the fibrotic-related gene expression. The results showed that Maculosin inhibits the activation of lung fibroblasts and attenuates the accumulation of extracellular matrix （ECM） induced by exogenous TGF- β2. Further study suggested that the anti-fibrotic activity of Maculosin may be related to repressing the expression of inflammatory cytokines and promoting the process of ECM degradation.

关 键 词：Maculosin 肺成纤维细胞 转化生长因子-Β 炎症因子 细胞外基质 

分 类 号：R285[医药卫生—中药学]