高速逆流色谱法分离纯化灯心草的四个菲类化合物  

Preparative Isolation and Purification of Four Phenanthrenes from Juncus effusus by HSCCC

作　　者：李贵云[1] 陈泽蔓 王小红[1] 黄建梅[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《天然产物研究与开发》2014年第8期1248-1251,共4页Natural Product Research and Development

基　　金：国家自然科学基金项目(81173541)

摘　　要：本研究建立了高速逆流色谱法分离纯化灯心草中四个主要菲类成分effusol、dehydroeffusol、juncusol、dehydrojuncusol的方法。以环己烷-乙酸乙酯-甲醇-水为溶剂体系,上相为固定相,下相为流动相,流速2mL/min,主机转速850 rpm,温度25℃,对灯心草经80%乙醇回流提取,乙酸乙酯超声萃取得到的粗提物经高速逆流色谱2次纯化,得到dehydroeffusol、effusol、dehydrojuncusol、juncusol的纯度分别为99.3%、98.5%、98、7%、97.4%。This study is to establish the high-speed counter-current chromatography method for purification of effusol, de- hydroeffusol,juncusol,dehydrojuncusol from Juncus effusus L A two-phase solvent system was composed of cyclohexane- ethyl acetate-methanol-water（S： 3： 5： 3） ,The upper phase was stationary phase while the lower as mobile phase,the flow rate was 2 mL/mln,the revolution speed was 850rpm,and the temperature was 25 ℃. The ethyl acetate extracts of Juncus effusus was purified by HSCCC twice. The purity of effusol,dehydroeffusol,juncusol,dehydrojuncusol was 99.3%, 98.5% ,98.5% ,97.4%.

关 键 词：灯心草 菲类成分 高速逆流色谱 分离 

分 类 号：R965.2[医药卫生—药理学]