合欢皮总皂苷对人微血管内皮细胞体外增殖的影响  

Effects of Albizzia julibrissin Durazz Saponins on Proliferation of Human Microvascular Endothelial Cells

作　　者：冯磊[1] 谭莹 陈爽 邱丽颖[1] 金坚 

机构地区：[1]江南大学无锡医学院 [2]江南大学药学院,无锡214122

出　　处：《天然产物研究与开发》2014年第8期1272-1275,共4页Natural Product Research and Development

基　　金：江苏省中医药管理局康缘中医药科技创新基金项目(HZ0816KY)

摘　　要：研究合欢皮总皂苷对人微血管内皮细胞增殖的药效学影响。采用人脐静脉微血管内皮细胞HMEC-1作为研究模型,经不同剂量合欢皮总皂苷作用48、72 h后,应用SRB法、光学显微镜观察合欢皮总皂苷对HMEC-1细胞增殖、形态的影响;应用台盼蓝染色法、DNA梯形条带法、流式细胞仪PI单染方法分析合欢皮总皂苷对HMEC-1细胞生长的影响。实验结果发现合欢皮总皂苷提取物具有抑制血管内皮细胞增殖的作用,其与时间、剂量均存在依赖关系,作用48 h的IC50为1.5μg/mL;合欢皮总皂苷使HMEC-1细胞周期阻滞在亚G0/G1期,高剂量作用48 h后可能引起细胞产生坏死。因此,合欢皮总皂苷可能通过改变细胞周期,部分引起细胞坏死,从而抑制人微血管内皮细胞的增殖。In this study,the effect of Albizziajullbrissin Durazz saponins on proliferation of microvascular endothelial cells （HMEC-1） was investigated. After 4B-hour and 72-hour intervention on HMEC-1 with different doses of A. julibrissin saponins ,HMEC-1 proliferation and morphology were observed by Sulforhodamine B staining and optical microscope. The effects of A. julibrissin saponins on HMEC-1 were also analyzed by Trypen blue staining, DNA ladder, PI staining and flow cytometer. The results showed that A. julibrissin sapenins affected HMEC-1 proliferation in time and dose-dependent manner,the ICsoof 48-hour intervention group was 1.5μg/mL. In addition,a block was found in G0/G1 stage of mitotic HMEC-1 cycle under the sapenins intervention, and cell necrosis in high dosage 48-hour intervention group. Above all, based on these results,it was concluded that cell necrosis was possibly due to cell cycle change under A. julibdssin sape- nins intervention, which inhibited HMEC-1 proliferation.

关 键 词：合欢皮总皂苷 人微血管内皮细胞 抑制增殖 细胞周期 

分 类 号：R932[医药卫生—生药学]