冬凌草活性部位逆转SGC7901/ADR细胞多药耐药性的体外研究  

Reversal of Multidrug Resistance in SGC7901 /ADR Cells by Rabdosia rubescens

作　　者：高巧慧 许文婷[1] 谢婷[1] 刘瑞媛[1] 吴臻[1] 李发荣[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,西安710062

出　　处：《天然产物研究与开发》2014年第8期1281-1284,共4页Natural Product Research and Development

基　　金：陕西省自然科学基金项目(2012JM4010);中央高校基本科研费项目(GK261001001)

摘　　要：以SGC7901和SGC7901/ADR为细胞模型,检测了冬凌草活性部位与化疗药物联用后,对SGC7901/ADR耐药性的逆转效应;冬凌草活性部位处理细胞后,检测耐药细胞内阿霉素的蓄积变化、耐药细胞P-糖蛋白(P-gp)的表达水平以及mdr1基因的表达变化。结果显示,冬凌草氯仿部位和乙酸乙酯部位可以有效提高化疗药物阿霉素在SGC7901/ADR细胞内的蓄积,降低P-gp的表达,降低mdr1基因的转录。冬凌草逆转胃癌耐药细胞SGC7901/ADR多药耐药性的活性部位是冬凌草氯仿部位和乙酸乙酯部位,其逆转作用与抑制P-gp的表达相关。The effect of Rabdosia mbescena on the expression and function of P-glycoprotein （P-gp） in SC, C7901/ADR ceil was investigated in this study. The potential bio-active extract fractions were selected to carry out experiments in SGC7901/ADR cells. After treatment with chloroform fraction and ethyl acetate fraction of R. mbescens,the cell viability was examined by MTT assay,the expression of P-gp in SC, C7901/ADR and SGC7901 cells was determined by fluores- cent-labeled antibody technique, and the expression of mdrl gene was detected by RT-PCR. The experimental resttlts indicated that chloroform fraction and ethyl acetate fraction of R. mbesceng increased the intracellular accumulation of adriamycin in SGC7901/ADR ceils, and decreased the expression of mdrl gene and P-gp. Therefore, chloroform fraction and ethyl acetate fraction of R. rubescena had effects on reversal of multidrug resistance of SGC7901/ADR ceils, and the mechanism was possibly associated with the inhibition of the expression of P-gp.

关 键 词：冬凌草 SGC7901 ADR 多药耐药 P-糖蛋白 

分 类 号：R285.5[医药卫生—中药学] Q946[医药卫生—中医学]