灵芝孢子副产物体外抗氧化活性的研究  

Antioxidant Activities of Extracts of By-products of Ganoderma lucidum Spores

作　　者：陈亚莉[1] 逄增润 张雯[1] 徐靖 王瑛 郑化先 

机构地区：[1]华东师范大学生命科学学院,上海200241 [2]浙江寿仙谷生物科技有限公司 [3]浙江省珍稀植物药工程技术研究中心,浙江321200

出　　处：《天然产物研究与开发》2014年第8期1303-1307,1293,共6页Natural Product Research and Development

基　　金：国家自然科学基金项目(61172149);浙江省产业技术创新战略联盟建设项目(2011LM201)

摘　　要：利用有机溶剂萃取法获得灵芝孢子副产物五个不同极性部位,以总抗氧化能力(T-AOC)及羟自由基(·OH)和2,2-二(4-叔辛基苯基)-1-苦肼基自由基(DPPH·)的清除能力为评价指标筛选最优抗氧化活性部位,并对其进行抗脂质过氧化研究,结果显示:不同极性部位均能清除上述自由基,其中乙酸乙酯相活性最强。进一步研究发现,乙酸乙酯相能够显著抑制β-胡萝卜素/亚油酸体系,同时对小鼠肝脏自氧化及H2O2诱导的肝脂质过氧化和红细胞过氧化溶血均有较好保护作用。实验表明:灵芝孢子副产物不同极性部位提取物均具一定程度的抗氧化活性,其中乙酸乙酯相活性最强。The objective of this study was to investigate the antioxidant activities of by-products of Ganoderma luc/dum spores. Ethanol soluble extract of by-products of Ganoderma luc/dum spores was prepared by 80% alcohol,followed by fractional extraction respectively with petroleum ether,chloroform,ethyl acetate and n-butanol. Then the antioxidant activities of above five different fractions were evaluated by T-AOC and free radical-scavenging power like DPPH · and · OH to identify the most efficient fraction. The results indicated that all fractions showed high radical scavenging efficiency,especially the ethyl acetate fraction （EA） which was chosen to take a further study on anti-lipid poroxidation. In this study, the protective effects of EA at 0.5 mg/mL on β-carotene/linoleic acid system was 70.32%. The inhibitory activity of EA at 2 mg/mL on hemolysis of erythrocytes and lipid peroxidation of mice homogenate induced by H2O2 were 41.39% and 51.00%. Taking together, each extract has significant antioxidant activities, especially the ethyl acetate extract.

关 键 词：灵芝孢子副产物 自由基 乙酸乙酯相 脂质过氧化 

分 类 号：R285[医药卫生—中药学]