响应面法优化铁棒锤根中一种咪唑生物碱的超声提取工艺  

Optimization of Ultrasonic-assisted Extraction of An Imidazole Alkaloid from Aconitum pendulum Busch by Response Surface Methodology

作　　者：刘永玲[1,2] 王萍[1,2] 陈涛[1] 陈晨[1] 李玉林[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008 [2]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2014年第8期1311-1315,共5页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目(KSCX-EW-J-26);青海省应用基础研究项目(2012-Z-715)

摘　　要：采用响应面法优化铁棒锤根中一种咪唑生物碱Imidazole-2-Carboxylic acid butyl ester(ICABE)的超声提取工艺。在单因素试验基础上筛选出液料比、提取温度、提取时间三个主要因素,并通过响应面分析得到的最佳工艺条件为:液料比23 mL/g、提取温度58℃、提取时间51 min,在此条件下的理论提取率为0.081%,实际测得值为0.079%,两者较接近。验证试验表明,所得模型方程能较好地预测试验结果。In this study, imidazole-2-carboxylic acid butyl ester （ICABE） was ultrasonically extracted from Aconitum pendulum Busch. Three influencing parameters, namely ratio of solvent to solid, extraction time and extraction temperature, were optimized using response surface methodology （RSM）. The optimum conditions were as follows：the ratio of solvent to solid was 23 mL/g,extraction temperature was 58 ℃ and extraction time was 51min. The predicted value of extraction rate of ICABE was 0. 081% under the optimum conditions, and the experimental value was 0.079%. The validation test showed that the ultrasonic-assisted extraction process of ICABE was successfully optimized by RSM.

关 键 词：铁棒锤 咪唑生物碱 超声提取 响应面法 

分 类 号：R284.2[医药卫生—中药学]