中华硬蜱TROSPA的发现与鉴定  

CONCEPTUAL TROSPA IDENTIFIED FROM IXODES SINENSIS （ACARI： IXODIDAE） IN SOUTH CHINA

作　　者：王欣[1] 孙毅[1] 曹务春[1] 蒋宝贵[1] 贾娜[1] 倪雪冰[1] 张圆[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《寄生虫与医学昆虫学报》2014年第2期123-128,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金项目（No.30872196;No.8271878）

摘　　要：笔者从我国莱姆病媒介蜱种中华硬蜱中鉴定出莱姆病螺旋体外膜蛋白A受体TROSPA。中华硬蜱幼蜱体内的两个cDNA克隆I．sin-1（258bp）和I．sin-2（462bp）经序列比对和分析属于TROSPA．其中，I．sin-2的氨基酸序列同肩板硬蜱I.scapularis、篦子硬蜱I.ricinus和全沟硬蜱I.persulcatus的相似度分别为88．2％、88．5％和88．8％，而I.sin-1则分别存在46％、46．1％和45．6％的相似度。中华硬蜱TROSPA的发现为解释了中华硬蜱传播莱姆病的机制以及研究中华硬蜱与病原体的相互关系奠定了基础。TROSPA, a kind of tick receptor for outer surface protein A （OspA） of Lyme spirochete, was identified from specific vector species, Ixodes sinensis in South China. To improve our standing of the role of tick gene in tick borne pathogen infection/multiplication, larva of I. sinensis were allowed to feed on mice, two cDNA clones including I. sin-1 （258 bp） and I. sin-2 （462 bp） were identified as conceptual TROSPA. The putative amino acid sequence of I. sin-2 was 88.2% , 88.5% and 88.8% identical to that of I. scapularis, I. ricinus and I. persulcatus respectively, while the predicted amino acid of I. sin-1 shows only 46% , 46. 1% , 45.6% to each respectively. This finding will lay the basis to analyze the interactions between I. sinensis and pathogens, and evaluate its potential application in blocking pathogen transmission from vector to host.

关 键 词：中华硬蜱 莱姆病螺旋体 外膜蛋白 TROSPA 

分 类 号：Q78[生物学—分子生物学]