固定化菌株DF51对土壤及大豆幼苗苯酚污染的修复  

Restoration of Immobilized Rhodococcus sp. DF51 on Soil and Soybean Seedlings Phenol Pollution

作　　者：张谨华[1] 王慧阳[1] 王鑫[2] 

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030600 [2]太原市星火技术发展中心,山西太原030009

出　　处：《山西农业科学》2014年第8期865-868,873,共5页Journal of Shanxi Agricultural Sciences

基　　金：山西省自然科学基金项目(2013011039-2)

摘　　要：试验采用随机区组设计,在大田条件下研究施用不同质量浓度的苯酚(0,50,100,150,200 mg/L)和固定化菌株DF51(0,10,20,30,40 kg/hm2)对土壤及大豆的影响。结果表明,苯酚显著降低了大豆的光合特性、抗逆性、产量;固定化菌株DF51对苯酚污染土壤具有修复性能,且能提高苯酚胁迫下大豆的光合特性、抗逆性及其产量。P1D1(50 mg/L苯酚,10 kg/hm2固定化菌株DF51)和P2D2(100 mg/L苯酚,20 kg/hm2固定化菌株DF51)处理可恢复苯酚胁迫下大豆的生理等特性。固定化菌株DF51能显著提高大豆在苯酚胁迫下的耐性,这与其提高抗氧化酶活性有关。To study the phenol soil pollution restoration of immobilized Rhodococcus sp. DF51 and its influence on soybean seedlings, randomized block design was used in the experiment. Soybean seedlings were treated with either different concentrations of phenol （0, 50, 100, 150, 200 rag/L） or immobilized Rhodococcus sp. DFS1 （030,20, 30,40 kg/hm2） or their combinations to test their effects on the growth, photosynthetic characteristic and resistance. The results show that the photosynthetic characteristic, resistance and yield were depressed under phenol treatment. It was found that immobilized Rhodococcus sp. DF51 could restore the phenol soil pollution. It was also found that the photosynthetic characteristic, resistance and yield were improved under immobilized Rhodococcus sp. DF51 for phenol pollution soil. The treatment with P1D1 or P2D2 could restore the physiological characteristics of the soybean under phenol stress. It was indicated that immobilized Rhodococcus sp. DF51 had a significant influence on endurance of soybean under the stress of phenol. These improvments were related with the increase of ant/oxidant enzymes.

关 键 词：固定化菌株DF51 苯酚 大豆 生理特性 产量 

分 类 号：TQ450.2[化学工程—农药化工]