HPLC法测定骨刺消痛胶囊中欧前胡素的含量  

Determination of Imperatorin in Gucixiaotong Capsule by HPLC

作　　者：任广菊 孙坤[1] 梁馨元[1] 姜一婧 夏丽杰[1] 

机构地区：[1]吉林省白山市食品药品检验所,白山134300

出　　处：《中国中医药现代远程教育》2014年第13期156-157,共2页Chinese Medicine Modern Distance Education of China

摘　　要：目的采用高效液相色谱法测定骨刺消痛胶囊中欧前胡素的含量。方法色谱柱:Thermo C18(4.6×250mm,5m),流动相:甲醇-水(54:46),流速:0.8ml·min^(-1),柱温:30℃,检测波长:300nm^([1])。结果欧前胡素在0.07595μg^0.45570μg范围内与峰面积呈良好的线性关系(r=0.9998);平均加样回收率为98.1%,RSD为0.2%(n=6)。结论该方法简便易行,结果可靠,可有效地控制骨刺消痛胶囊的质量。Objective Determination of imperatorin in gucixiaotong capsule by HPLC. Methods chromatographic column： Thermo C18 （4.6×250mm, 5 μm）, mobile phase ： methanol-water （54：46）, flow rate： 0.8ml.min-1, column temperature： 30 ℃, detection wavelength： 300nm. Results Irnperatorin in 0.07595 μg--0.45570μg range and peak area had a good linear relationship （r=0.9998）; the average recovery was 98.1%, RSD was 0.2% （n=6）. Conclusion The method is simple, reliable, and can effectively control the quality for gucixiaotong capsules.

关 键 词：骨刺消痛胶囊 高效液相色谱法 白芷 欧前胡素 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]