心脏营养素-1对大鼠损伤脊髓的保护作用  

Protective effect of exogenous cardiotrophin-1(CT-1)against spinal cord injury in rats

作　　者：李菁[1] 朱磊[1] 林浩东[1] 陈爱民[1] 

机构地区：[1]第二军医大学长征医院创伤与修复重建外科,上海200003

出　　处：《第二军医大学学报》2014年第7期703-707,共5页Academic Journal of Second Military Medical University

基　　金：上海市教育委员会人才计划项目(XYQ020111021)~~

摘　　要：目的探讨心脏营养素-1(cardiotrophin-1,CT-1)对大鼠损伤脊髓的保护作用,并初步探讨其可能的作用机制。方法 90只大鼠随机分为9组:脊髓损伤前正常大鼠(0h)以及脊髓损伤模型建立后1h、6h、12h、1d、2d、3d、6d、12d组(n=10),RT-PCR测定损伤脊髓组织CT-1及其受体糖蛋白130(gp130)、白血病抑制因子受体(LIFR)mRNA表达。大鼠随机分为3组:CT-1干预组、模型组和神经生长因子(NGF)干预组(n=30),分别给予rhCT-1(100μg·kg-1·d-1)或等量生理盐水或NGF硬膜内灌注,给药后第3天、1周、2周于损伤脊髓处取材行H-E和尼氏染色,对比分析各组神经元计数。大鼠随机分为模型组及CT-1干预组(n=30),损伤后1、4、12周完整剥离大鼠小腿三头肌(n=10),比较两组肌肉质量及总蛋白量。结果与损伤前(0h)相比,大鼠脊髓损伤后CT-1mRNA表达出现一过性增高(1h,P<0.05),而后呈进行性下降趋势;其受体表达出现反馈性增高。CT-1干预组、NGF干预组神经元及尼氏体数量均高于模型组(P<0.05),前两组间差异无统计学意义。脊髓损伤后4周、12周时CT-1干预组大鼠小腿三头肌湿质量及总蛋白含量均高于模型组(P<0.05)。结论 CT-1对大鼠脊髓损伤后神经元存活及功能维持具有一定的保护作用。Objective To investigate the protective effect of exogenous cardiotrophin-1（CT-1）against spinal cord injury and the possible mechanism.Methods Ninety rats were evenly randomized into 9 groups,namely,before injury（0h）and 1,6,12 hours,1,2,3,6 and 12 days after injury.RT-PCR was used to examine the expression of CT-1,its receptor gp130,and leukemia inhibitory factor receptor（LIFR）in the injured spinal cord tissues.Rats were also divided into spinal cord injury model group,nerve growth factor（NGF）treatment group and CT-1 treatment group,receiving intradural infusion of normal saline,NGF,and rhCT-1（100μg·kg-1·d-1）,respectively;the injured tissues were harvested at the 3rd day,one week and 2 weeks after injury for H-E and Nissl staining;and the neuron counts were compared between different groups.Sixty rats were divided into model group and CT-1 treatment group（n=30）;the triceps muscle wet weight and total protein weight were compared between the two groups at 1 week,2 weeks and 4 weeks after spinal cord injury.Results CT-1mRNA expression had a transient significant increase after injury（P0.05）,and then it decreased gradually;meanwhile,expression of its receptors increased consequently.The numbers of neurons and Nissl bodies were similar in CT-1-treated group and NGF-treated group,but the numbers of both groups were significantly more than that in the model control group（P0.05）.In addition,the muscle wet weight and protein content in the CT-1-treated group were significantly higher than that in the model control group at 4 weeks and 12 weeks after injury（P0.05）.Conclusion CT-1 exhibits a protective effect on the survival and function of neurons after spinal cord injury in rats.

关 键 词：脊髓损伤 心肌营养素-1 神经营养因子 神经保护 

分 类 号：R651.21[医药卫生—外科学]