不同途径移植骨髓间充质干细胞治疗兔肝硬化模型效果比较  

Comprison among curative effects of different transplantion of BMSCs on rabbit model of cirrhosis

作　　者：李晨[1] 宫卫东[1] 李晓冰[1] 吴智群[1] 

机构地区：[1]第四军医大学唐都医院介入放射科,陕西西安710038

出　　处：《实用放射学杂志》2014年第7期1210-1213,1250,共5页Journal of Practical Radiology

基　　金：陕西省社发攻关课题资助项目（2009k17-05）;中科院“西部之光”基金资助项目.

摘　　要：目的：探讨骨髓间充质干细胞（bone marrow stem cells,BMSCs）通过不同移植途径对治疗兔肝硬化模型疗效的差别。方法用四氯化碳液体橄榄油混合液皮下注射和乙醇喂养的方法建立肝硬化兔模型,将肝硬化模型随机分为肝硬化模型组（n=10）、肝动脉移植组（n=10）、耳静脉移植组（n=10）。取健康新西兰大耳兔骨髓,利用全骨髓贴壁的方法原代培养骨髓 BMSCs,然后通过3种途径分别进行移植并检测血清白蛋白、转氨酶；通过 H E染色观察肝硬化程度以及利用Western-Blotting 法检测血清白蛋白在肝脏中的表达。结果分别通过静脉组与动脉组移植 BMSCs 到兔肝硬化模型中,4周后发现移植组模型兔的肝功能均得到明显改善,血清白蛋白较肝硬化未移植组显著升高（P〈0.05）,转氨酶较肝硬化未移植组显著降低（P〈0.05）。2种途径移植BMSCs对兔肝硬化的治疗效果有统计学差异（P〈0.05）。结论 BMSCs 移植通过耳静脉和肝动脉治疗肝硬化兔4周后,肝功能都有明显的改善。但经肝动脉移植治疗效果明显优于经静脉移植治疗效果。Objective To investigate the effect of bone marrow mesenchymal stem cells （bone marrow stem cells,BMSCs） through different approaches in the treatment of transplanted cirrhotic model in rabbit effect difference.Methods Establishment of rabbit model of carbon tetrachloride cirrhosis with liquid olive oil mixture of subcutaneous inj ection and ethanol fed,the liver cirrho-sis models were randomly divided into cirrhosis model group （n=10）,hepatic artery transplantation group （n=10）,ear vein trans-plantation group （n=10）.Healthy New Zealand rabbits bone marrow,using the method of whole bone marrow adherent cultured bone marrow BMSCs,and then were transplanted and serum albumin,alanine aminotransferase in 3 ways;by HE staining,the de-gree of hepatic cirrhosis and detection of serum albumin by Western blot expression in liver.Results Through the vein group and ar-tery transplantation group BMSCs to cirrhotic model in rabbit,4 weeks after the discovery of liver transplantation group rabbits were significantly improved,serum albumin is liver cirrhosis without transplantation group increased significantly（P〈0.05）,transami-nase is liver cirrhosis without transplantation group decreased significantly （P〈0.05）.The therapeutic effect of 2 ways of BMSCs transplantation on rabbit liver cirrhosis had a significant difference （P〈0.05）.Conclusion Transplantation of BMSCs through the ear vein and the hepatic artery of rabbit after 4 weeks of treatment of liver cirrhosis,liver function can be improved.But the hepatic artery transplantation therapy is obviously better than that of intravenous transplantation of treatment effect.

关 键 词：骨髓间充质干细胞 兔 肝硬化模型 

分 类 号：R332[医药卫生—人体生理学] R575.2[医药卫生—基础医学]