肺炎链球菌生物膜形成厚度与青霉素最低抑菌浓度的关系  

The relationship between Streptococcus pneumoniae biofilm formation and antibiotic resistance

作　　者：刘青[1] 施毅[1] 朱素华[1] 高卫卫[1] 孙禾[1] 苏欣[1] 

机构地区：[1]南京大学医学院附属金陵医院(南京军区南京总医院)呼吸与危重症医学科,南京医学硕士研究生210002

出　　处：《医学研究生学报》2014年第7期690-693,共4页Journal of Medical Postgraduates

基　　金：中国呼吸道感染优化治疗协作组科研专项基金(2009037)

摘　　要：目的肺炎链球菌可形成生物膜。文中旨在研究青霉素不同最低抑菌浓度(minimal inhibitory concentration,MIC)的肺炎链球菌生物膜形成厚度的差别。方法收集2010年11月至2012年10月南京军区南京总医院、江苏省人民医院、江苏省中医院、南京医科大学第二附属医院、南京市鼓楼医院、东南大学附属中大医院、南京市第一医院等7家医院共147株肺炎链球菌,琼脂稀释法测定青霉素MIC,根据MIC分为≤0.065μg/mL组、0.5μg/mL组、2μg/mL组及≥4μg/mL组,每组选择4珠菌,共16珠菌;96孔板、24孔板培养生物膜24h,分别在570nm处测A值并且激光共聚焦扫描显微镜(confocal laser scanning microscope,CLSM)观察生物膜形成。结果生物膜半定量检测及CLSM显示所有菌株都形成生物膜。≤0.065μg/mL组平均A值(0.228±0.063)高于0.5μg/mL组(0.200±0.061)和≥4μg/mL组(0.186±0.050),差异均有统计学意义(P<0.05),但与2μg/mL组(0.203±0.049)差异无统计学意义(P>0.05);0.5μg/mL组、2μg/mL组≥4μg/mL组A值两两比较的差异无统计学意义(P>0.05)。结论青霉素MIC高的肺炎链球菌生物膜厚度有减弱的趋势,生物膜的形成是复杂的过程,受多种因素影响。Objective Streptococcus pneumoniae can form biofilms .The aim of this study was to investigate the biofilm forma-tion of Streptococcus pneumoniae and the relationship with antibiotic resistance of penicillin etc . Methods A total of 147 clinical iso-lates of Streptococcus pneumoniae were collected from 7 teaching hospitals in Nanjing from 2010 to 2012.Minimal inhibitory concentration （MIC） of penicillin, erythromycin, cefuroxime and ceftriaxone were determined by agar dilution method .Streptococcus pneumoniae with various penicillin MIC was selected randomly as follow：MIC≤0.065μg/mL, 0.5μg/mL, 2μg/mL and≥4μg/mL, which was incuba-ted to form biofilms in 96-well plates and 24-well plates for 24 hours.The A values at 570 nm was measured and the biofilm was observed through confocal laser scanning microscope （ CLSM） . Results The biofilm semi-quantitative detection and CLSM both displayed that all strains formed biofilms.The A value of the group which penicillin MIC was ≤0.065μg/mL （0.228 ±0.063） was higher than the 0.5μg/mL group （0.200 ±0.061） and the≥4μg/mL group （0.186 ±0.050） （P〈0.05） , and there was no difference among the groups which penicillin MIC were 0.5μg/mL, 2μg/mL and≥4μg/mL, respectively （P〉0.05）.The A value of the group which erythromycin MIC was ≤0.5μg/mL （0.211 ±0.068） was higher than the ≥4μg/mL group （0.201 ±0.052） （P〉0.05）.The A value of the group sensitive to cefuroxime （0.216 ±0.062） was higher than the group resistant to cefuroxime （0.196 ±0.054） （P〈0.05）. Conclusion Streptococcus pneumoniae can form biofilms .Streptococcus pneumoniae with high antibiotics MIC has a trend of weakened biofilm formation .

关 键 词：肺炎链球菌 生物膜 最低抑菌浓度 激光共聚焦扫描显微镜 

分 类 号：R978.11[医药卫生—药品]