生长分化因子-9对牛卵丘细胞增殖的影响  

Effect of Growth Differentiation Factor-9 (GDF-9) on Bovine Cumulus Cells Proliferation

作　　者：欧阳效晴[1] 张春强[1] 栗瑞兰[1] 张通[1] 范晓梅[1] 高丽霞[1] 赵濛[1] 张家新[1] 

机构地区：[1]动物遗传育种与繁殖自治区重点实验室内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《中国细胞生物学学报》2014年第7期920-926,共7页Chinese Journal of Cell Biology

基　　金：国家科技支撑计划(批准号:2011BAD19B01);国家自然科学基金(批准号:31060307);公益性行业(农业)科研专项经费(批准号:201003061)资助的课题~~

摘　　要：研究生长分化因子-9(growth differentiation factor-9,GDF-9)对牛卵丘细胞增殖的影响。采用MTT法检测不同浓度GDF-9对卵丘细胞增殖的影响,结果表明,GDF-9能促进卵丘细胞的增殖,且GDF-9与卵丘细胞增殖效应存在浓度梯度关系;在卵丘细胞增殖过程中,FSH在一定程度上与GDF-9发挥协同作用。在GDF-9和FSH的作用下,去除卵母细胞的卵丘细胞复合体(oocytectomized cumulus cell complexes,OOX)也可以保持较好的发育形态。实时定量PCR结果表明,随着GDF-9浓度的增加,卵丘细胞扩展相关基因PTX3、HAS2及PTGS2的表达量也增加。总之,以上的研究结果表明,GDF-9可以促进卵丘细胞的增殖,对卵丘细胞功能的发挥起着重要的作用。This paper investigated the effect of growth differentiation factor-9 （GDF-9） on the proliferation of bovine cumulus cells. The effect of GDF-9 with different concentration on cumulus cells proliferation was studied by MTT assay. MTT results showed that GDF-9 could promote cumulus cell proliferation by a concentration-dependent manner, and FSH had a synergistic effect with GDF-9 on inducing cumulus cell proliferation. In addition, GDF-9 combined with FSH could promote oocytectomized cumulus complexes （OOX） development. The expression of cumulus cell expansion-related genes PTX3, HAS2 and PTGS2 were examined by Real-time quantitative PCR. The results showed that the expression of expansion-related gene were up-regulated by GDF-9. In conclusion, GDF-9 can promote cumulus cells proliferation and play an important role in regulating function of cumulus cells.

关 键 词：牛卵丘细胞 扩展相关基因 增殖 生长分化因子-9 

分 类 号：Q25[生物学—细胞生物学]