经胸腔镜建立巴马小型猪慢性心肌缺血模型  

Establishment of a Bama Miniature Swine Model of Chronic Myocardial Ischemic Models by Thoracoscopy

作　　者：张波[1] 陈保富[1] 马德华[1] 王益兵[2] 叶敏华[1] 夏利龙[1] 陈宇[1] 王斌[3] 王铮[4] 朱成楚[1] 

机构地区：[1]浙江省台州医院心胸外科,317000 [2]浙江省台州医院麻醉科,317000 [3]浙江省台州医院心内科,317000 [4]浙江省台州医院核医学科,317000

出　　处：《医学研究杂志》2014年第7期104-107,共4页Journal of Medical Research

基　　金：浙江省实验动物科技计划项目(2011C37083)

摘　　要：目的本研究目的在于探索采用胸腔镜微创技术建立小型猪慢性心肌缺血模型。方法选用实验用巴马小型猪,采用胸腔镜技术,在左冠状动脉回旋支(left circumflex coronary artery)主干放置Ameroid缩窄环,术后第6周经选择性冠状动脉造影,了解冠脉狭窄程度及侧支循环形成并行心脏核素显像了解心肌缺血情况,以对胸腔镜下建立小型猪慢性心肌缺血模型进行评估。结果造模成功率80%(8/10),手术时间45.3±7.1min,术中出血量32.5±11.3ml。术后第6周造影显示6头左回旋支完全闭塞,见侧支循环形成,2头狭窄程度大于70%。心肌灌注显像见左后下壁明显缺损。结论采用胸腔镜方式应用Ameroid缩窄环建立小型猪慢性心肌缺血模型技术微创、可行,模型均一、稳定,适用于慢性心肌缺血模型相关实验研究。Objective To explore the method to establish miniature swine model of chronic myocardial ischemia using thoracoscopic minimally invasive techniques.Methods A ameroid constrictor was placed around the proximal of left circumflex coronary artery （LCX）in Bama miniature swine by thoracoscopy.The chronic myocardial ischemia was formed by gradual occlusion of the LCX）.Six weeks later,coronary artery angiography （CAG） was performed to evaluate the degree of stenosis.Single-Photon Emission Computed Tomography was used to assess myocardial perfusion.Results The success rate of modeling was 80 ％ （8/10）.The operation time was 45.3 ± 7.I min,and the amount of bleeding was 32.5 ± 11.3ml.Six weeks later,the CAG analysis showed the total occlusion of the LCX in 6swines,stenosis degree ＞70％ in 2 swines.SPECT showed the obvious myocardial perfusion defects in the left rear wall.Concluion Thoracoscopic minimally invasive techniquesh can be successfully used to construct miniature swines chronic myocardial ischemic models with less injury.This way represents a useful tool for the chronic myocardial ischemia model relevant experimental study.

关 键 词：慢性心肌缺血 小型猪 胸腔镜 

分 类 号：R33[医药卫生—人体生理学]