S100A10在人类月经周期子宫内膜中的表达变化  

Cyclic changes of S100A10 expression in human endometrium

作　　者：尉晓蔚[1] 佟少明[2] 燕秋[3] 

机构地区：[1]大连大学附属中山医院外科实验室,116001 [2]辽宁师范大学生命科学院 [3]大连医科大学基础医学院

出　　处：《中华医学杂志》2014年第27期2152-2155,共4页National Medical Journal of China

摘　　要：目的 观察S100A10在人类月经周期子宫内膜中的表达变化.方法 采用免疫组织化学、免疫印迹以及实时定量PCR检测人类月经周期子宫内膜中S100A10蛋白和基因的表达及定位.结果 40例人类子宫内膜标本结果显示,在增殖期S100A10蛋白的表达较弱,主要表达于子宫内膜腔上皮和腺上皮细胞的细胞质和细胞膜上,在基质细胞有散在表达;在分泌期的各时期,S100A10蛋白水平明显高于增殖期的各时期（2.26±0.17、3.75±0.22、2.65±0.20比1.17±0.11、1.32±0.14,均P＜0.05）,并且在着床窗口期的表达呈现峰值;到分泌晚期略有下降（P＜0.05）.基因检测结果显示,分泌期各期S100A10的mRNA的表达明显高于增殖期各期（0.50±0.05、1.02±0.10、0.55±0.05比0.25±0.02、0.30±0.03,均P＜0.05）,在分泌中期出现表达峰值.结论 S100A10可能在胚胎植入中发挥作用,可以作为子宫内膜接受态的潜在标志物之一.Objective To explore the cyclic changes of S100A10 expression in human endometrium.Methods The gene and protein expression of S100A10 in human endometrium was examined by real-time polymerase chain reaction （PCR）,immunohistochemical staining and Western blot.Results S100A10 protein was predominantly expressed in cytoplasm and cytomembrane of luminal and glandular epithelia.The S100A10 protein was continuously expressed from proliferative phase to secretory phase of menstrual cycle.However the expression in the secretory phase was much higher than in the proliferative phase （2.26 ±0.17,3.75 ±0.22,2.65 ±0.20 vs 1.17 ±0.11,1.32 ±0.14,both P ＜0.05）.Specifically,the highest level of S100A10 expression occurred during the mid-secretory phase.Then realtime PCR was used to analyze the mRNA expression of S100A10 in human endometrial samples at various points during the menstrual cycle.And the results confirmed the corresponding changes at the genetic level （0.50 ±0.05,1.02 ±0.10,0.55 ±0.05 vs 0.25 ±0.02,0.30 ±0.03,both P ＜0.05）.Conclusion The endometrial expression of S100A10 increases during the window of embryo implantation.It suggests that it may increase the endometrial receptivity of uterine epithelial cells for embryos.

关 键 词：S100A10蛋白 月经周期 子宫内膜 标志物 

分 类 号：R339.2[医药卫生—人体生理学]