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出 处:《职业与健康》2014年第16期2318-2319,共2页Occupation and Health
基 金:中央补助地方禽流感监测项目(项目编号:2011-2013年)
摘 要:目的掌握郧县活禽市场禽流感病毒分布现况,为疫情风险评估防控提供依据。方法2011—2013年每季度采集10—15份4个活禽市场外环境(禽笼、禽粪、冲洗水)标本,用荧光定量RT—PCR方法检测禽流感病毒FluA、AH5、AH9、ANl核酸。结果2011—2013年FluA、AH5、AH9、ANl平均检出率为46.25%、13.13%、35.63%、6.25%,2011年AH5检出率(27.59%)高于AH9(13.79%),2012和2013年AH5检出率(1.67%、16.90%)低于AH9(31.67%、47.89%);F1uA检出率第2~4季度高于第1季度(χ2=8.665,P〈0.01),AH9检出率第2—4季度高于第1季度(χ2=6.372,P〈0.05),AH5仅在2和4季度检出;冲洗水和禽笼的FluA、AH5、AH9检出率分别高于禽粪(χ2=6.240,P〈0.05、X2=5.432,P〈0.05、χ2=11.684,P〈0.01);在冲洗水中检出AH5和ANl同时阳性8份,在禽笼中检出AH5和ANl同时阳性2份。结论郧县活禽市场外环境存在禽流感病毒AH9、AH5等亚型污染,应重点加强该环境监测,重点做好职业暴露人群的预防。[ Objective] To know the distribution status of avian influenza virus in live poultry markets in Yunxian County, provide the basis for risk assessment and control of epidemic situation. [ Methods] 10-15 external environment samples (poultry cage, poul- try excrement and washing water } were collected from 4 live poultry markets in every quarter from 2011-2013. [ Results ] During 2011-2013, the average detection rate of FluA, AHS, AH9 and AN1 was 46.25%, 13.13%, 35.63% and 6.25%, respectively. The detection rate of AH5 ( 27.59% ) in 2011 was higher than that of AH9 ( 13.79% ), while the detection rates of AH5 in 2012 and 2013 ( 1.67% and 16.90% } were lower than those of AH9 (31.67% and 47.89% ). The detections rate of FluA in the sec- ond, third and forth quarter was higher than that in the first quarter ( χ2 = 8. 665, P 〈 0. 01 ) , the detection rate of AH9 in the sec- ond, third and forth quarter was higher than that in the first quarter ( χ2 = 6. 372, P 〈 0. 05 ) , and AH5 was found only in the second and forth quarter. The detections rates of FluA, AH5 and AH9 in washing water and poultry cage were higher than those in poultry excrement (χ2= 6.240, P 〈 0.05 ; Xz = 5. 432, P 〈 0.05 ; χ2= 11. 684, P 〈 0.01). AH5 and AN1 were simultaneously found in 8 samples of washing water, and 2 samples of poultry cage. [ Conclusion] The pollution of avian influenza virus ( AH9 and AH5 ) has been found in external environment of live poultry markets in'Yunxian County. It is necessary to strengthen the environ- mental monitoring, and focus on the prevention of occupational exposure population.
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