神经生长因子促进海马胆碱能神经再生过程中Lhx8的动态表达  

Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration

作　　者：李浩明[1] 朱培培[1] 金国华[1] 施金洪[1] 邹琳清[1] 田美玲[1] 衣昕[1] 秦建兵[1] 

机构地区：[1]南通大学医学院人体解剖学系江苏省神经再生重点实验室,江苏南通226001

出　　处：《解剖学报》2014年第4期441-445,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(31070937);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的探讨神经生长因子(NGF)促进海马胆碱能神经再生与同源盒基因Lhx8之间的关系。方法SD大鼠72只,在制备切割右侧穹隆海马伞模型和NGF侧脑室注射的基础上,分为对照组、切割组、NGF组、切割联合NGF组;Real-time PCR和Western blotting分别检测各组动物海马中Lhx8蛋白的表达变化;免疫荧光技术检测各组动物海马齿状回颗粒下层中胆碱乙酰转移酶(ChAT)/Lhx8双标细胞数。结果切割组和NGF组中Lhx8蛋白的表达量较对照组增高,切割联合NGF组增高更为明显,且Lhx8基因表达也增高;NGF组和切割联合NGF组中海马齿状回颗粒下层(SGZ)中的ChAT/Lhx8双标细胞数也较对照组和切割组明显增多。结论侧脑室注射NGF促进海马胆碱能神经再生与Lhx8的高表达有关。Objective To investigate the relationship between the nerve growth factor （ NGF ） induced hippocampal neuroregeneration and homeobox gene Lhx 8.Methods Seventy-two SD rats were divided into control group , transected group, NGF group, transected combined with NGF group after right fimbria-fornix transection and NGF intracerebroventricular injection . Real-time PCR and Western blotting were applied to detect the gene and protein expression of Lhx8 in each group.The choline acetyltransferase （ ChAT）/Lhx8 double labeled cells in subgranular zone （ SGZ） of hippocampus in each group were detected by immunofluorescence .Results The expression of Lhx8 gene and protein in the transected , NGF group and especially in the transected combined with NGF group was obviously higher than in the control group .The number of ChAT/Lhx8 double labeled cells in the NGF group and the transected combined with NGF group was obviously more than in the control group and transected group . Conclusion The hippocampal neuroregeneration which induced by NGF intracerebroventricular injection was associated with the higher expression of Lhx8.

关 键 词：Lhx8 海马 神经生长因子 胆碱能神经 再生 实时定量聚合酶链反应 大鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]