NonO蛋白在丁酸钠诱导小鼠红白血病细胞分化过程中的表达  

Expression of NonO protein during murine erythroleukemia cell differentiation induced by sodium butyrate

作　　者：桑婷婷[1] 胡江江 薛建有[1] 戚武林 赵辅昆[1] 张世馥[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《解剖学报》2014年第4期516-520,共5页Acta Anatomica Sinica

摘　　要：目的探讨NonO蛋白在丁酸钠诱导小鼠红白血病(MEL)细胞向红细胞系分化过程中的表达变化。方法利用联苯胺染色观察丁酸钠诱导MEL细胞红细胞系分化情况,通过Western blotting、免疫细胞化学检测NonO蛋白在丁酸钠诱导MEL细胞分化的过程中表达量的变化,PCR技术检测NonO基因在RNA水平上的表达变化,免疫细胞化学技术检测NonO蛋白在MEL细胞中的定位。结果发现NonO在丁酸钠诱导MEL细胞向红细胞系分化过程中在基因水平和蛋白水平上均上调表达,NonO蛋白在MEL细胞中定位于细胞质和细胞核。结论NonO蛋白与丁酸钠诱导MEL细胞分化密切相关。Objective To study NonO protein expression changes in murine erythroleukemia （ MEL ） cell differentiation induced by sodium butyrate .Methods Benzidine staining was used to test sodium butyrate-induced erythroid differentiation of MEL cells .We detected NonO protein expression changes in MEL cell differentiation induced by sodium butyrate and NonO protein localization in MEL cells by Western blotting and immunocytochemistry .Furthermore , we applied PCR technique to detect NonO RNA expression in differentiation process .Results We found that NonO protein was upregulated at gene and protein levels in the erythroid differentiation process of MEL cells induced by sodium butyrate and located in cytoplasm and nucleus in MEL cells .Conclusion These results show that NonO protein is closely related with MEL cell differentiation induced by sodium butyrate , which may provide important clues for further study of the mechanism of leukemia .

关 键 词：NonO蛋白 小鼠红白血病细胞 丁酸钠 红细胞系 分化 免疫组织化学 免疫印迹法 

分 类 号：R552[医药卫生—血液循环系统疾病]