外源性音猥因子对成年大鼠脊髓损伤后室管膜区神经干细胞增殖的影响  

Effects of exogenous sonic hedgehog on proliferation of neural stem cells in ependymal area after spinal cord injury in adult rats

作　　者：马延超[1] 董海涛[1] 王翠芳[1] 张海鸿[1] 康学文[1] 夏亚一[1] 董平[1] 

机构地区：[1]兰州大学第二医院骨科,甘肃省骨与关节疾病重点实验室,730030

出　　处：《中华创伤杂志》2014年第7期740-743,共4页Chinese Journal of Trauma

基　　金：甘肃省自然科学基金资助项目（096RJZA076）

摘　　要：目的 研究外源性音猥因子（sonic hedgehog,Shh）对成年大鼠脊髓损伤（spinal cord injury,SCI）后室管膜区神经干细胞（neural stem cells,NSCs）的增殖及运动功能恢复的影响. 方法 雌性SD大鼠55只,任意选取5只作为正常对照组,余50只采用改良Allen打击法于T1o节段制作大鼠急性SCI模型,按随机数字表法分为Shh组和SCI组,每组25只.分别于伤后1,3,7,14,28 d采用改良Tarlov评分评价大鼠后肢运动功能恢复程度;免疫荧光双标染色观察大鼠SCI后Brdu和Nestin双阳性细胞数量的变化情况. 结果 术后7d起,Shh组和SCI组Tarlov评分比较差异有统计学意义（P＜0.05）.免疫荧光双标染色显示,Shh组自术后3d起各时相点Brdu/Nestin 双阳性细胞数量分别为97.20±18.23,153.60±25.76,133.80±21.02,89.80±15.42,较SCI组72.60±15.60,112.20±23.63,94.20±18.70,43.40±10.62明显增多（P均＜0.05）.结论 外源性Shh可促进成体大鼠SCI伤后室管膜区NSCs增殖以及运动功能的恢复.Objective To investigate effects of exogenous sonic hedgehog （Shh） on proliferation of neural stem cells （NSCs） in ependymal area and recovery of motor function after spinal cord injury （SCI） in adult rats.Methods Fifty-five female SD rats were involved in the study：five were selected as normal control group and fifty as Shh group （n =25） and SCI group （n =25） after being subjected to SCI at T10 segment using the modified Allen＇ s method according to the random number table.At 1,3,7,14,and 28 days after operation,restoration of hindlimb motor function of SD rats was assessed with modified Tarlov scale and changes of double positive cells of Brdu and Nestin with double-stained immunofluorescence.Results Tarlov scale revealed statistical difference between Shh and SCI groups since days 7 postoperatively （P ＜ 0.05）.In the double-staining test,number of double positive cells of Brdu and Nestin was greater in Shh Group than in SCI Group since day 3 postoperatively [（97.20 ± 18.23） vs （72.60± 15.60）,（153.60 ±25.76） vs （112.20 ±23.63）,（133.80 ±21.02） vs （94.20± 18.70）,（89.80 ± 15.42） vs （43.40 ± 10.62）,P ＜ 0.05].Conclusion Exogenous Shh is conducive to the proliferation of ependymal NSCs and the recovery of motor function in SCI rats.

关 键 词：脊髓损伤 干细胞 音猥因子 

分 类 号：R651.2[医药卫生—外科学]