Generating rats with conditional alleles using CRISPR/Cas9  

作　　者：Yuanwu Ma Xu Zhang Bin Shen Yingdong Lu Wei Chen Jing Ma Lin Bai Xingxu Huang Lianfeng Zhang 

机构地区：[1]Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medi- cal Sciences, Beijing 100021, China [2]MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing Uni versity, Nanjing Biomedical Research Institute, National Resource Center for Mutant Mice, Nanjing, Jiang Su 210061, China

出　　处：《Cell Research》2014年第1期122-125,共4页细胞研究（英文版）

摘　　要：Dear Editor, The rat is an important laboratory model and has many advantages over mouse models especially in toxicol- ogy and pharmacology studies. Several genome-editing technologies, such as zinc-finger nucleases （ZFNs） [1-2], transcription activator-like effector nucleases （TALENs） [3] and the Clustered Regularly Interspaced Short Palin- dromic Repeats （CRISPR）/CRISPR-associated 9 （Cas9） system [4-5],

关 键 词：基因组 大鼠 编辑技术 实验模型 小鼠模型 转录激活 核酸酶 药理学 

分 类 号：Q78[生物学—分子生物学] Q959.837