成年大鼠盆神经节神经元的体外培养与鉴定  

Culture and Identification of Adult Rat Major Pelvic Ganglia Neurons in Vitro

作　　者：程时刚[1] 钟伟[2] 张伊凡[1] 耿红琼[1] 彭荣[1] 杨星海[1] 

机构地区：[1]湖北省妇幼保健院外科,湖北武汉430070 [2]湖北省肿瘤医院乳腺科,湖北武汉430079

出　　处：《武汉大学学报（医学版）》2014年第4期546-549,共4页Medical Journal of Wuhan University

摘　　要：目的:探索成年大鼠盆神经节(MPG)神经元的体外培养、鉴定的实用方法。方法:分别利用植块培养法、分离细胞培养法对成年大鼠MPG神经元进行体外培养,应用免疫组化以神经微丝单克隆抗体(NF-200)对MPG神经元进行鉴定。结果:通过上述两种方法均能成功培养出生长状态良好的MPG神经元,细胞呈NF-200反应阳性。结论:本实验成功建立了成年大鼠MPG神经元的体外培养方法,可作为神经科学研究的重要手段。Objective. To establish a practicable procedure for the culture of major pelvic ganglia （MPG） neurons from adult rat in vitro. Methods. The MPG neurons were cultured in vitro by both explant culture method and dissociated cell culture method. MPG neurons were identified by immunostained with a neuron sp were immunoreactive to th ecific antibody, neurc e antibody again for the culture of MPG neurons from adult the field of neurology. st NF rat in filament-200 （NF-200）. Results： -200. Conclusion： We developed vitro, which might be useful for These a new neurons protocol the research in

关 键 词：成年大鼠 盆神经节 植块培养 分离细胞培养 

分 类 号：R694.5[医药卫生—泌尿科学]