穿心莲内酯对小鼠放射性肺损伤的防护作用  

Andrographolide protects against radiation-induced lung injury in mice

作　　者：康亚辉[1] 王金凤[1] 张曲[1] 黄关宏[1] 马建新[1] 杨百霞[2] 何向锋[3] 王忠明[3] 

机构地区：[1]江苏省连云港市第二人民医院放疗科,222000 [2]江苏省南通市肿瘤医院放疗科 [3]江苏省南通市肿瘤医院肿瘤内科

出　　处：《中华放射医学与防护杂志》2014年第7期507-511,共5页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的观察穿心莲内酯对C57BL/6小鼠放射性肺损伤的保护效应.方法将80只C57BL小鼠完全随机分为0.9％氯化钠溶液组（空白对照组）、穿心莲内酯组（单纯给药组）、放射＋0.9％氯化钠溶液组（单纯照射组）和放射＋穿心莲内酯组（联合组）,每组20只.照射前单纯给药组和联合组用穿心莲内酯20 mg·kg^-1·d^-1灌胃,空白对照组和单纯照射组用等体积0.9％氯化钠溶液灌胃,连续灌胃30 d.采用6 MV高能X射线直线加速器单次15 Gy照射,建立小鼠全肺放射性肺损伤模型.取小鼠肺组织HE染色、Masson染色进行组织病理学观察;采用ELISA法检测小鼠血清转化生长因子β1（TGF-β1）、肿瘤坏死因子α（TNF-α）;紫外分光光度计检测肺组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力和羟脯氨酸（Hyp）含量.结果联合组小鼠肺组织损伤程度较单纯照射组小鼠明显减轻,其肺系数、肺组织MDA含量、羟脯氨酸含量以及血清TGF-β1、TNF-α表达水平虽然略高于空白对照组,但低于单纯照射组差异有统计学意义（t肺系数=1,60,tMDA=7.06,t羟脯氨酸=17.44,tTGF-β1=16.67,t TNF-α=14.03,P＜0.05）;联合组小鼠血清SOD活力明显高于单纯照射组（t =60.81,P＜0.05）,但低于空白对照组和单纯给药组;单纯给药组小鼠各项检测指标与空白对照组相比,差异均无统计学意义.结论 穿心莲内酯可有效减轻小鼠的放射性肺损伤,且对小鼠肺组织无明显毒性.Objective To investigate the protective effect of andrographolide against radiation-induced lung injury （RILI） in C57BL/6 mice.Methods Eighty C57BL mice were randomly divided into four groups：un-irradiated and normal saline-treated group （n =20,control group）,un-irradiated and andrographolide-treated group （n =20,drug group）,radiation plus normal saline-treated group （n =20,radiation group） and radiation plus andrographolide-treated group （n =20,treatment group）.Before radiation,the mice in drug group and treatment group were administered daily via gavage with andrographolide （20 mg· kg-1 · d-1）） for 30 d,while the same volume of normal saline solution was given daily in the control and radiation groups.The model of RILI in C57BL mice was established by irradiating whole mouse chest with a single dose of 15 Gy of 6 MV X-rays.The pathological changes of the lung stained with HE/Masson were observed with a light microscope.The transforming growth factor-β1 （TGF-β1） and tumor necrosis factor-α （TNF-α） in serum were examined by enzyme-linked immunosorbent assay.The activities of malondialdehyde （MDA） and superoxide dismutase （SOD） and the content of hydroxyproline in lung tissues were examined by corresponding kits.Results Compared with radiation group,there was an obvious amelioration in pathological injury of lung tissue in the treatment group.The lung coefficient,the activities of lung tissue MDA,the content of Hyp,the serum content of hydroxide free radical,and the serum levels of TGF-β1 and TNF-α in the treatment group were significantly lower than those in radiation group at 24th week,（tlungcoefficient =1.60,tMDA =7.06,tHyp =17.44,tTGF-β1 =16.67,tTNF-α =14.03,P ＜ 0.05）,while slightly higher than those in control group.The activity of SOD was significantly higher in the treatment group than that in radiation group （t =60.81,P ＜ 0.05）,while lower than those in control group and drug group.There were no significant differences in these indicators be

关 键 词：穿心莲内酯 放射性肺损伤 小鼠 

分 类 号：R285.5[医药卫生—中药学]