2011年我国部分地区猪瘟病毒分子流行病学研究  

Molecular Epidemiology of Classical Swine Fever Virus in some Regions of China in 2011

作　　者：朱长康[1,2] 徐璐[2] 范学政[2] 陈锴[1,2] 张正兴[2] 郑然[2] 赵燕[2] 徐志文[1] 宁宜宝[2] 王琴[2] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]中国兽医药品监察所猪瘟参考实验室,北京海淀100081

出　　处：《中国兽医杂志》2014年第7期3-5,I0001,共4页Chinese Journal of Veterinary Medicine

基　　金：国家科技支撑计划(2006BAD06A18;2006BAD06A03)

摘　　要：本研究对2011年我国部分地区养猪场采集的疑似猪瘟病料组织样本209份,分别用FQ-RT-PCR和RT-nPCR两种方法检测猪瘟病毒（CSw）.结果FQ-RT-PCR检测出55份猪瘟阳性,RT-nPCR检测出53份阳性,2种方法符合率达96.3％.将53份阳性产物进行测序及同源性分析,显示这53份流行株均为2.1基因亚型.所分离的流行株与疫苗株（HCLV）和石门株（SM）的核营酸同源性分别为79.4％～83.1％和79.4％～82.5％.研究结果表明,目前我国部分地区的养殖场仍有猪瘟病毒的存在,且以2.1亚型为主;流行株与疫苗株同源性仍高于77.4％,结合免疫攻毒试验,说明目前使用的猪瘟疫苗依然有效.Two hundreds and nine suspected classical swine fever virus infected samples were collected from different regions of China in 2011. All of these samples were detected by FQ-RT-PCR and RT-nPCR. Fifty-five samples were positive as detected by FQ-RT-PCR, but 53 positive by RT-nPCR. The coincidence rate of the two methods is 96.3%. All of the positive products were sequenced and analyzed. The results showed that all of the field isolates were in subgroup 2.1. The nucleotide homology was 79.4% 83.1% compared with HCLV and was 79.4%-82.5% compared with SM strain. It was found that CSFV are still prevalent in some regions of China and most of the field isolates are in subgroup 2.1. HCLV is still a very good vaccine for control of CSF because nucleotide homogeneity between field isolates and HCLV is higher than 77.4%.

关 键 词：猪瘟病毒 E2基因 分子流行病学 

分 类 号：S852.651[农业科学—基础兽医学]