萨罗罗非鱼AQP3 cDNA序列克隆及盐度胁迫下组织表达特征  

cDNA Cloning of Aquaporin 3 in Sarotherodon melanothern and Its Tissue Expression Patterns under Salinity Stress

作　　者：甘远迪[1] 赵金良[1] Jeerawat Thammaratsuntorn 李传阳[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306

出　　处：《动物学杂志》2014年第4期560-569,共10页Chinese Journal of Zoology

基　　金：现代农业产业技术体系专项(No.CARS-49-4B);上海高校知识服务平台上海海洋大学水产动物遗传育种中心项目(No.ZF1206)

摘　　要：利用cDNA末端快速扩增法(RACE)克隆了萨罗罗非鱼(Sarotherodon melanothern)鳃组织中水通道蛋白3(AQP3)的cDNA序列。AQP3 cDNA全长1894 bp,其中,开放阅读框912 bp,编码303个氨基酸,5'和3'非编码区长度分别为98 bp和884 bp。氨基酸序列分析显示,萨罗罗非鱼AQP3与莫桑比克罗非鱼(Oreochromis mossambicus)同源性最高,达94%,含6个跨膜区。应用实时荧光定量PCR技术(qRT-PCR)检测了不同盐度胁迫下萨罗罗非鱼11种组织中AQP3 mRNA的相对表达水平。0、15盐度下,鳃、肌肉、皮肤中表达水平相对较高,其他组织表达相对较低,且15盐度中各组织的表达水平低于0盐度;30盐度下,各组织以肠道表达量相对最高。推测在不同的渗透压调节作用中,萨罗罗非鱼通过不同组织器官中AQP3来参与水的转运过程。To understand the role of aquaporin 3（ AQP3） in osmoregulatory processes of Sarotherodon melanothern,the complete cDNA sequence of AQP3 was cloned from gills of S. melanothern using rapid amplification of cDNA ends（ RACE）. The full length of AQP3 cDNA was 1 894 bp,containing a 912 bp open reading frame encoding 303 amino acids,with a 98 bp 5’ untranslated region（ UTR） and a 884 bp 3’-UTR（ Fig. 1）. The amino acid sequence comparison showed that AQP3 of S. melanothern shared the highest identity with Oreochromis mossambicus（ 94%）,and it processed six transmembrane domains（ Fig. 3）.Relative tissue expression levels of AQP3 mRNA under different salinity stresses were estimated by real-time quantitative PCR. Higher expression was detected in skin,gill and muscle than in other tissues at 0 and 15salinity; moreover,the expression level in 15 salinity was lower than that in freshwater in all tissues（ Fig. 5）.The expression level was higher in intestine than in other tissues at 30 salinity（ Fig. 5）. The results suggest S.melanothern can utilize AQP3 in different tissues to respond to water transfer under different osmoregulationprocesses.

关 键 词：萨罗罗非鱼 水通道蛋白3 CDNA 组织表达 盐度胁迫 

分 类 号：Q78[生物学—分子生物学]