Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system  

作　　者：Pei Zhao Zhe Zhang Hongmei Ke Yiren Yue Ding Xue 

机构地区：[1]School of Life Sciences, Tsinghua University, Beijing 100084, China [2]Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA

出　　处：《Cell Research》2014年第2期247-250,共4页细胞研究（英文版）

摘　　要：Dear Editor, Technologies to achieve specific and precise genome editing, such as knock-in and knock-out, are critical for deciphering the functions of a gene and for under- standing fundamental biological processes. Compared with the zinc finger nucleases （ZFN） and transcription activator-like effector nucleases （TALEN）, which have been used for genome editing [1], the Clustered Regu- larly Interspaced Short Palindromic Repeats （CRISPR）/ CRISPR-associated （Cas） system has emerged as a new powerful tool for genome modifications. It has recently been adopted for genome editing in human cell lines [2- 4], mouse [5], zebrafisb [6], C. elegans [7-12], and plants [13].

关 键 词：靶基因 编辑 寡核苷酸 线虫 系统 基因组 基因敲除 生物过程 

分 类 号：Q78[生物学—分子生物学] G232[文化科学]