IL-6对小鼠肾上腺皮质细胞系Y1合成皮质醇的影响  

Effects of IL-6 on corticosteroid synthesis of mouse adrenal cortex cell line Y1

作　　者：文小平[1] 夏海鸣[2] 

机构地区：[1]东南大学医学院,江苏南京210009 [2]东南大学附属第二医院,江苏南京210003

出　　处：《东南大学学报（医学版）》2014年第4期456-460,共5页Journal of Southeast University(Medical Science Edition)

基　　金：南京市科技发展计划基金资助项目(20091093);南京市卫生局医学重点科技发展基金资助项目(ZKX08033)

摘　　要：目的:探讨白介素6(IL-6)对小鼠肾上腺皮质细胞系Y1合成皮质醇能力及合成过程中类固醇急性调节蛋白(StAR)相关基因表达的影响。方法:100 ng·ml-1IL-6单独或联合10-9mol·L-1促肾上腺皮质激素(ACTH)处理Y1细胞后,台盼蓝拒染法检测细胞活力,放免法检测细胞皮质醇合成量,实时荧光定量PCR法检测StAR mRNA表达水平。结果:IL-6单独或联合ACTH处理Y1细胞后,皮质醇合成量增加(P<0.05),同时StAR mRNA表达水平上升(P<0.05)。结论:IL-6可单独或联合ACTH通过促进StAR mRNA表达增强Y1细胞合成皮质醇的能力。Objective： To explore the effects of interleukin-6 （IL-6） on synthesis of corticosteroid in mouse adrenal cortex cell lines Y1 （Ylcells） and the steroid acute regulatory protein（STAR） related gene. Methods： The Y1 ceils were treated with 100 ng ·ml^-1 IL-6 alone or combined with 10^-9 mol ·L^-1 adrenocorticotropic hormone （ACTH）. The cell viability was detected by blue dye exclusion method. Cortieosteroid synthesis level of the cells was determined by radioimmunoassay. The change of StAR mRNA was tested by real-time PCR. Results： After treated with IL-6 or/and ACTH, synthesis of corticosteroid had been increased and StRA mRNA expression was significantly increased（ P 〈 0.05 ）. Conclusion： IL-6 alone or combined with ACTH could promote the synthesis of corticosteroid by increasing StAR mRNA expression.

关 键 词：白介素6 Y1细胞 皮质醇 细胞色素P450侧链裂解酶 

分 类 号：R586[医药卫生—内分泌]