Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis  

作　　者：Lan-Tao Gou Peng Dai Jian-Hua Yang Yuanchao Xue Yun-Ping Hu Yu Zhou Jun-Yan Kang Xin Wang Hairi Li Min-Min Hua Shuang Zhao Si-Da Hu Li-Gang Wu Hui-Juan Shi Yong Li Xiang-Dong Fu Liang-Hu Qu En-Duo Wang Mo-Fang Liu 

机构地区：[1]Center for RNA Research, State Key Laboratory of Molecular Biology-University of Chinese Academy of Sciences, Shanghai 200031, China [2]Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Insti- tutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [3]Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, Guangdong 510275, China [4]Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0651, USA [5]National Population and Family Planning Committee Key Laboratory of Contraceptive Drugs & Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China [6]Department of Biochemistry and Molecular Biology and Center for Genetics and Molecular Medicine, School of Medicine, University of Louisville, Louisville, KY40202, US

出　　处：《Cell Research》2014年第6期680-700,共21页细胞研究（英文版）

基　　金：We thank Drs Narry Kim （Seoul National University, Korea） and Fatitima Gebauer （Centre for Genomic Regulation, Spain） for suggestions and critical comments on the manuscript; Drs Chen Chu, Junhao Li, and Xianghua Piao for experimental and bioinformatic assistance. This work was supported by grants from the Ministry of Science and Technology of China （2011CB811303, 2014CB964802, 2014CB943103, 2012CB910803）, the National Natural Science Foundation of China （31325008, 91219306, 31270840, 31170754, 31300656）, and Science and Technology Commission of Shanghai Municipality （13ZR 1464300）.

摘　　要：Spermatogenesis in mammals is characterized by two waves of piRNA expression： one corresponds to classic piR- NAs responsible for silencing retrotransponsons and the second wave is predominantly derived from nontransposon intergenic regions in pachytene spermatocytes, but the function of these pachytene piRNAs is largely unknown. Here, we report the involvement of pachytene piRNAs in instructing massive mRNA elimination in mouse elongating spermatids （ES）. We demonstrate that a piRNA-induced silencing complex （pi-RISC） containing murine PIWI （MIWI） and deadenylase CAF1 is selectively assembled in ES, which is responsible for inducing mRNA deadenylation and decay via a mechanism that resembles the action of miRNAs in somatic cells. Such a highly orchestrated program appears to take full advantage of the enormous repertoire of diversified targeting capacity of pachytene piRNAs de rived from nontransposon intergenic regions. These findings suggest that pachytene piRNAs are responsible for inactivating vast cellular programs in preparation for sperm production from ES.

关 键 词：pachytene piRNAs MIWI CAF1 pi-RISC mRNA deadenylation ES 

分 类 号：Q78[生物学—分子生物学] Q954.434