Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system  

作　　者：Pengpeng Liu Lijiang Long Kai Xiong Bo Yu Nannan Chang Jing-Wei Xiong Zuoyan Zhu Dong Liu 

机构地区：[1]The Education Ministry Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-membrane and Membrane Bio-engineering, School of Life Sciences, Peking University, Beijing, China [2]Institute of Molecular Medicine, Peking University, Beijing, China

出　　处：《Cell Research》2014年第7期886-889,共4页细胞研究（英文版）

基　　金：Acknowledgments We are grateful to Drs M Dong and M Ding for their critical comments. We thank the Caenorhabditis elegans Genetic Center for providing additional worm strains and greatly appreciate comments from and proof-reading by Drs R Peterson and L Tao. Thisproject has been supported by the National Basic Research Program of China （973 Program; 2011CBA01102 and 2012CB944503 to DL, 2012CB944501 to J-W X）, the National Natural Science Foundation of China （90919034 to DL） and the Peking-Tsinghua Center for Life Sciences （to DL）. PL was a recipient of the PKU President Graduate Scholarship.

摘　　要：Type II clustered, regularly interspaced, short palindromic repeat （CRISPR）-associated （Cas） system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA （sgRNA） or Cas9-sgRNA ribonucleoproteins . In the present study, we have developed a CRIS- PR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA （gRNA） through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner.

关 键 词：编辑工具 基因组 系统 线虫 遗传 核糖核蛋白 转基因方法 培养细胞 

分 类 号：Q78[生物学—分子生物学] TP391[自动化与计算机技术—计算机应用技术]