三种制作Balb/c小鼠下肢缺血模型方法的比较  

Comparison of three approaches to establishing Balb/c mouse models of hind-limb ischemia

作　　者：陈永林[1] 张景科[2] 孙少华[1] 

机构地区：[1]兰州大学第一医院病理科,甘肃兰州730000 [2]兰州大学实验动物中心,甘肃兰州730000

出　　处：《南方医科大学学报》2014年第8期1167-1170,共4页Journal of Southern Medical University

摘　　要：目的采用高位结扎并离断一段股动脉、单纯性高位结扎和单纯性低位结扎股动脉3种术式,制作Balb/c小鼠下肢缺血模型。通过比较3种术式的优缺点,为制作用于再生医学研究的缺血模型提供理论依据。方法选8周龄的雄性Balb/c小鼠30只,随机分成3组,分别按3种方法制作缺血模型。术后连续观察5 d。第2天行激光多普勒检测。结果激光多普勒显示3种模型中手术侧的下肢血流阻滞。高位结扎并离断股动脉组中小鼠整个肢体坏疽并脱落;单纯性高位结扎组小鼠中出现小腿及脚爪坏疽;单纯性低位结扎小鼠脚掌干燥并有脱皮现象,个别脚爪坏死,组织学见大片肌组织坏死及再生俢复反应。结论单纯性低位结扎股动脉是制作Balb/c小鼠下肢缺血模型比较好的方法。Objective To compared 3 approaches to establishing Balb/c mouse models of hind-limb ischemia suitable for studies of regenerative medicine. Methods Thirty eight-week-old male Balb/c mice were randomly divided into 3 groups for establishing models of hind-limb ischemia by both ligating and transecting the femoral artery proximal to the origin of the deep femoral artery branch, ligating the femoral artery proximal to the origin of the deep femoral artery branch, or ligating the femoral artery distal to the femoral artery trunk. The mice were continuously observed for 5 days after the operation, and hind-limb blood perfusion was monitored by laser Doppler imaging on the second postoperative day. Results All the 3 surgical approaches caused blood flow block in the hind-limbs of the mice. In mice with ligation and transaction of the femoral artery proximal to the origin of the deep femoral artery branch, dry gangrene occurred in the whole hind-limb. Dry gangrene also occurred in the legs and paws of mice with ligation of the femoral artery proximal to the origin of the deep femoral artery branch. In mice with femoral artery ligation distal to the origin of the deep femoral artery branch, the sole of the foot was dry with scaling skin and necrosis of several digits; microscopically examination revealed a large quantity of necrotic muscles accompanied by muscle regeneration. Conclusion Ligating the femoral artery distal to the origin of the deep femoral artery branch can be optimal for establishing Balb/c mouse models of hind-limb ischemia.

关 键 词：下肢缺血 结扎 BALB C小鼠 血管再生 

分 类 号：R543[医药卫生—心血管疾病]