布鲁氏菌A、M因子血清的研制  

Preparation of Monospecific Serum against Brucella A and M Epitopes

作　　者：张金亚[1] 程君生[1] 冯宇[2] 丁家波[1] 王楠[1] 皮向成 吴竞[3] 张东东[1] 朱良全[1] 毛开荣[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]山东农业大学动物科技学院,山东泰安271018 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国兽药杂志》2014年第8期1-5,共5页Chinese Journal of Veterinary Drug

基　　金：国家高技术研究发展计划("863"计划)(2012AA101302);北京市科技新星计划(XX2013099)

摘　　要：研制牛种布鲁氏菌A因子血清和羊种布鲁氏菌M因子血清，用于光滑型布鲁氏菌特异性种属鉴定。将灭活牛种布鲁氏菌2308株及羊种布鲁氏菌16M株免疫1．5～2 kg家兔各5只制备高免血清。对高免血清采用抗原交叉吸附的方法，制得A、M因子血清，分装冻干后置-20℃保存。微量试管凝集试验结果显示：研制的A因子血清对16M抗原在1∶2．5时无凝集现象，对2308抗原凝集价为1∶320；研制的M因子血清对2308抗原在1∶2．5时无凝集现象，对16M抗原凝集价为1∶160。玻片凝集试验结果显示：研制的A、M因子血清对异种抗原无凝集现象。试验表明，研制的A、M因子血清具有特异性强、效价高的特点，可辅助光滑型布鲁氏菌特异性种属鉴定。In order to identify different smooth Brucella strains, we prepared the monospecific serum against B.abortus O-chain A epitope and B.melitensis O-chain M epitope. Hyperimmune serum was collected from five rabbits, which were inoculated with heat-inactivated B.abortus （2308 strain） and heat-inactivated B.melitensis （16M strain） respectively. The hyperimmune serum was treated by serum antigen cross-absorption to get monospecific antiserum. Monospecific serum against B. abortus O-chain A epitopes were obtained which at a 1/320 dilution agglutinated the homologous antigen and at a 1/2. 5 dilution did not agglutinated the the heterologous antigen. Monospecific serum against B. melitensis O-chain M epitopes were obtained which at a 1/160 dilution agglutinated the homologous antigen and at a 1/2.5 dilution did not agglutinated the the heterologous antigen. Then the monospecific serum was lyophilized and stored at-20℃. The slide agglutination test showed that monospecific serum against B. abortus O-chain A epitope and B. melitensis O-chain M epitope had no agglutination with homologous antigen. The results showed that the obtained monospecific serum were of high specificity and high titer, which could assist the identification of smooth Brucella species.

关 键 词：布鲁氏菌 A因子血清 M因子血清 交叉吸收 

分 类 号：S852.614[农业科学—基础兽医学]