影响注射用白油佐剂免疫效果的因素分析  

Analysis about the Influence Factors of Injection White Oil Adjuvant on Immune Effects

作　　者：武桂梅[1] 刘纯德 王振辉[1] 慈洪波 刘军[3] 

机构地区：[1]吉林农业科技学院制药工程学院,吉林吉林132101 [2]吉林市吉化江城油脂化工有限公司,吉林吉林132012 [3]吉林正业生物制品股份有限公司,吉林吉林132101

出　　处：《中国兽药杂志》2014年第8期18-22,共5页Chinese Journal of Veterinary Drug

基　　金：吉林省教育厅"十二五"科研项目(吉教科合字[2011]第276号)

摘　　要：为分析影响白油佐剂免疫效果的主要因素,在前期研究基础上,参照国内大量进口白油GW1的馏分烃类组成及含量,制备出3批白油(JH)。以免疫效果较好的国内白油(GN1、GN2)和进口白油(GW1、GW2)作参照,首先分析了白油样品的馏分烃类组成含量和正碳数分布数据;然后分别制备新城疫灭活疫苗进行免疫,检测免疫鸡血清抗体效价和药物残留情况。结果显示,白油样品杂质含量由高到低的顺序是GW2>GN2>GW1>GN1>JH2>JH3>JH1,免疫效果由高到低的顺序是JH2>GW1>JH1>GN2>GN1>GW2>JH3。试验表明,影响白油佐剂免疫效果的主要因素是总芳烃含量和重金属含量,其次是白油的运动黏度。To analyze the influence factors of injection white oil adjuvant on immune effects, 3 batches of injection white oil （ JH） were prepared on basis of preliminary studies and by reference to large amount of the composition as well as the content of distillate hydrocarbon types in foreign GW1 white oil. Firstly we took the national injection white oil （ GN2, GN1 ） which had better immune effects and imports injection white oil （ GW1, GW2 ） as reference and analysed the content of distillate hydrocarbon types, data about carbon number distribution of injection white oil samples. Then inavtivated newcastle disease vaccine were prepared for immunization effect test to detect the serum antibody titer and the drug residues of the immune chicken. It turned out that the amount of impurityes in white oil samples ranged from high to low, the order was GW2〉GN2〉GN〉GW1〉GN1〉JH2〉JH3〉JH1. The immune effects order from high to weak was JH2〉GW1〉JH1〉GN2＆〉GN1〉GW2〉JH3. Experimental analysis concludes that the main factors affecting the white oil adjuvant effect are the content of total aromatic and the heavy mental concentrations, the kinematic viscosity of the white oil is less related.

关 键 词：注射用白油 馏分烃类 总芳烃 影响因素 

分 类 号：S852.4[农业科学—基础兽医学]