ω-3多不饱和脂肪酸对严重烧伤大鼠肾组织及其炎性反应的影响  

Effects of ω-3 polyunsaturated fatty acids on kidney and inflammatory response in burned rats

作　　者：葛章伟 蔡晨[1] 徐庆连[1] 潘浩斌[1] 

机构地区：[1]安徽医科大学第一附属医院烧伤科,安徽合肥230022

出　　处：《肠外与肠内营养》2014年第4期233-235,共3页Parenteral & Enteral Nutrition

基　　金：安徽省自然科学基金资助项目(090413267X)

摘　　要：目的:研究ω-3多不饱和脂肪酸(ω-3 PUFA)对严重烧伤大鼠肾组织及其炎性反应的影响。方法:将72只大鼠随机分成三组,即假烫组(A组)8只、对照组(中长链脂肪酸组,B组)32只和治疗组(ω-3 PUFA组,C组)32只。采用大鼠30%总体表面积(TBSA)Ⅲ度烫伤模型。B组每天按时经尾静脉注射与C组相同热量的中长链脂肪酸,C组经尾静脉注射ω-3 PUFA 2 ml/(kg·d),A组不做任何处置。分别观察A、B、C组在伤后第1、4、7和10天的血清前列腺素E2(PGE2)、白细胞介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)水平。采用普通聚和酶链反应(RT-PCR)检测肾组织TNF-αmRNA的表达。各个时间点均处死8只大鼠。结果:B组和C组大鼠在伤后各时间点血清PGE2、IL-1和TNF-α水平显著高于A组(P<0.01)。C组血清PGE2、IL-1和TNF-α水平低于B组,在伤后第4、7和10天与B组比,差异有显著性统计学意义(P<0.05,P<0.01)。另外,B组和C组大鼠在伤后各时间点肾组织TNF-αmRNA表达较A组明显升高。与B组比较,C组肾组织TNF-αmRNA的表达明显降低。结论:ω-3 PUFA能降低烧伤后大鼠血清PGE2、IL-1和TNF-α水平,减轻烧伤后肾组织TNF-αmRNA的表达,可能对烧伤后肾组织具有保护作用。Objective：To study the influence of ω-3 polyunsaturated fatty acids （ω-3 PUFA） on kidney and inflammatory reaction in serious scalded rats.Methods：A total of 72 SD rats with 30％ total body surface area （TBSA） third-degree scalded were separated into 3 groups：the phony scald group （group A）8,the control group （middle-long chain fatty acids group,group B） 32 and treatment group （ω-3 PUFA group,group C）32.Rats in group B were injected middle-long chain fatty acids with equivalent calories by tail vein,and rats in group C were injected ω-3 PUFA 2 mL/（kg · d） by tail vein on time for everyday.Rats in Group A had no any disposal.The levels of serum prostaglandin E2 （PGE2）,Interleukin-1 （IL-1）and tumor necrosis factor alpha levels （TNF-α） were examined.Expression of kidney tissue TNF-α mRNA was measured by RT-PCR technology.Results：Serum PGE2 、IL-1 、TNF-α were significantly higher than pre-injury at each time point after injury in group B ＆ C （P ＜ 0.01）.The levels in group C were lower than in group B,and were significantly different in 4 d,7 d,10 d after injury （P ＜0.05 or P ＜0.01）.Compared with group B in4d,7d after injury,the expression of kidney tissue TNF-α mRNA was significantly reduced in group C.Conclusion：ω-3 PUFA can reduce the levels of serum PGE2,IL-1,TNF-α,relieve the expression of kidney tissue TNF-α mRNA,and protect the damage of kidney after bum.

关 键 词：烧伤 不饱和脂肪酸 肿瘤坏死因子-α 白细胞介素-1 前列腺素E2 肾 

分 类 号：R644[医药卫生—外科学] R392[医药卫生—临床医学]