次声激活小胶质细胞抑制成年大鼠海马神经干细胞增殖  

Involvement of microglial activation in infrasound-induced suppression of neural stem cell proliferation in adult rat hippocampus

作　　者：董正[1] 蔡婧[2,3] 林甜[2,4] 史明[2] 

机构地区：[1]第四军医大学学员旅12队,北京100101 [2]第四军医大学西京医院神经内科,北京100101 [3]第四军医大学西京医院内分泌科,西安710032 [4]解放军第306医院神经内科,北京100101

出　　处：《神经解剖学杂志》2014年第4期408-412,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31170801)

摘　　要：目的:探讨次声对成年大鼠海马齿状回颗粒细胞下层(subgranular zone,SGZ)神经干细胞增殖抑制作用的细胞学机制。方法:成年雄性Sprague-Dawley大鼠置于次声压力舱,连续暴露于16 Hz、130 dB次声7 d(2h/d)后,给与小胶质细胞抑制剂米诺环素(50 mg/kg,药物组,n=16)或等体积生理盐水(对照组,n=16),同时设立不经次声作用的正常对照组(n=16);分别于1、3、7和14 d处死大鼠,利用免疫组织化学法以Iba1、OX42标记小胶质细胞,BrdU标记增殖的神经干细胞。结果:小胶质细胞在SGZ区分布较为密集;与正常对照组相比,次声暴露后3 d时OX42免疫反应性明显增强、SGZ区BrdU阳性细胞数目减少最为明显(P<0.01);米诺环素可显著改善次声暴露后BrdU阳性细胞数目的减少(P<0.01)。结论:小胶质细胞活化参与次声抑制成年大鼠海马SGZ区神经干细胞的增殖。Objective： To explore the cellular mechanisms underlying infrasound-induced suppression of neural stem cell proliferation in the subgranular zone （SGZ） of hippocampal dentate gyrus of adult rats. Methods： Male Sprague-Dawley rats were placed in a infrasonic chamber and exposed to 16 Hz at 130 dB infrasound for 2 h/d in consecutive 7 days. Then rats were treated with microglia inhibitor minocycline （ the drug group, 50 mg/kg, n = 16） or same volume of saline （ the control group, n = 16） intraperitoneally. The rats without infrasound exposure and drugs treatment were established as the normal control group （ n = 16）. At 1,3, 7 or 14 d following infrasound exposure, the rats were sacrificed and their brain sections were subjected to immunostaining of Iba1, OX42 （ two markers for mieroglia）, and BrdU （ a marker for proliferating cells）. Results： Microglia was distributed abundantly in the SGZ region. Relative to the normal controls, at 3 d following infrasound exposure, OX42 immunoreactivity was most enhanced and BrdU-positive cells were decreased to the lowest number in the SGZ （ P 〈 0.01 ）. Minocycline attenuated the infrasound-induced decrease of BrdU-positive cell number significantly （P 〈0.01 ）. Conclusion： Microglial activation was involved in infrasound-induced suppression of neural stem cell proliferation in hippocampal SGZ of adult rats.

关 键 词：次声 海马齿状回 神经干细胞 小胶质细胞 细胞增殖 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]