SOX2/Nestin阳性细胞在糖尿病大鼠穹窿下器中的表达  

The expression of SOX2 Nestin positive cells in subfornical organ of diabetic rat

作　　者：唐蔚东[1] 李彩格[2] 叶建亚[1] 郝庆卯[1] 秦永[1] 李力[1] 马常升[3] 

机构地区：[1]河北医科大学西山校区,石家庄050000 [2]河北医科大学第二医院内分泌科,石家庄050000 [3]河北医科大学基础医学研究所神经生物学研究室,石家庄050000

出　　处：《神经解剖学杂志》2014年第4期437-441,共5页Chinese Journal of Neuroanatomy

基　　金：河北省高等学校科学研究计划项目(Q2012082);河北省卫生厅医学科学研究资助项目(20120045)

摘　　要：目的:观察神经干细胞特异性标记物性别决定基因高迁移率组蛋白(SOX2)、巢蛋白(nestin)在不同时期糖尿病大鼠穹窿下器(SFO)中的表达情况并探讨其意义。方法:雄性Wistar大鼠给予链脲佐菌素(STZ,60mg/kg,腹腔一次性注射)建立Ⅰ型糖尿病大鼠模型。用免疫组织化学染色方法观察SOX2、Nestin阳性表达细胞在正常大鼠及2、4、8、12周糖尿病大鼠SFO的表达情况。结果:模型组各个时间点的SOX2和Nestin的阳性表达细胞数量及平均光密度均高于正常对照组,且均在第4周表达达到高峰,而后逐渐降低,差异有统计学意义(P<0.05)。结论:在不同病程糖尿病大鼠模型中,SOX2及Nestin在SFO的阳性表达一过性增强,表明糖尿病时可能会短暂性提高SFO神经干细胞的增殖功能。Objective： To observe the expression of sex-determining genes in high-mobility group protein （SOX2） and nestin in subfornical organ （SFO） of diabetic rats over different time course and the implicated significance. Methods： Male Wistar rats received intraperitoneal injection of streptozotocin （ STZ, 60 mg,/kg） to make Type I diabetic rat model. The expression of SOX2 and nestin positive cells in SFO of normal rats and diabetic rats at 2, 4, 8 and 12 weeks post injection were observed by immunohistochemical staining. Results： There were more positive cells and higher optical density of SOX2 and Nestin in the model group than in matched group, and the number reached the peak level at 4 weeks and then gradually decreased, showing statistical significance （P 〈 0.05）. Conclusion： The expression of SOX2 and nestin were transiently enhanced in SFO, indicating that diabetes mellitus may transitorily enhance proliferating capacity of neural stem cell in SFO.

关 键 词：穹窿下器 神经干细胞 SOX2 NESTIN 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]