鸡胚后脑内背侧抑制性轴突导向蛋白的表达与23C10阳性神经元的发育相关  

Correlation of draxin expression with the normal development of 23C10 positive neurons in hindbrain of chick embryo

作　　者：王合梅[1] 崔慧先[2] 岂春晓[2] 王磊[2] 康林[2] 苏玉红[2] 

机构地区：[1]河北医科大学附属第四医院麻醉科,石家庄050000 [2]河北医科大学人体解剖学教研室,石家庄050017

出　　处：《神经解剖学杂志》2014年第4期442-446,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31100789);教育部高等学校博士学科点专项科研基金(20111323120006);河北省自然科学基金(C2011206032)

摘　　要：目的:初步探讨背侧抑制性轴突导向蛋白即draxin在鸡胚后脑23C10阳性神经元发育过程中的作用。方法:应用免疫组化检测不同发育阶段正常鸡胚后脑内draxin蛋白的表达与23C10阳性神经元及其轴突的发育情况;应用活组织切片与draxin-AP融合蛋白体外孵育的方法,检测23C10阳性神经元及其轴突是否具有与draxin蛋白在体外直接结合的能力。结果:HH18-26发育阶段的鸡胚后脑内,draxin蛋白主要分布在基底节的封套层内;在相同的发育阶段,23C10阳性神经元则在后脑基底节封套层内形成并与周围结构形成大量的轴突联系;大量23C10阳性神经元及其轴突具有与draxin蛋白在体外直接结合的能力。结论:鸡胚后脑内draxin蛋白参与调控早期23C10阳性神经元的形成及其与其他细胞间的轴突投射。Objective： To investigate the role of the repulsive axon guidance protein, draxin, in the development of 23C10 positive neurons in the hindbrain of chick embryo. Methods： Immunohistochemistry was used to observe the expression of draxin protein and the development of 23C10 positive neurons and axons in the chick embryonic hindbrain; Draxin-AP fusion protein binding with living hindbrain sections was performed to check whether 23C10 positive neurons and axons can bind draxin protein directly. Results： At HH18-26 developmental stage, draxin protein was distributed to the mantle zone of the basal plate of the chick hindbrain. At the same developmental stages, lots of 23C10 positive neurons were formed inside the mantle zone of the basal plate of the chick hindbrain. These cells formed axonal connection with surrounding structures widely. Lots of 23C10 positive neurons and axons had the ability to bind draxin protein directly in vitro. Conclusion： Draxin was involved in the regulation of early formation of 23C10 positive cells and of the axonal projection between 23C10 positive ceils and other cells inside chick hindbrain region.

关 键 词：背侧抑制性轴突导向蛋白 后脑 23C10阳性神经元 轴突 鸡胚 

分 类 号：R338[医药卫生—人体生理学]