禽支气管炎病毒Jin-13分离株在SPF鸡体内的动态分布  

Dynamic Distribution of The Avian Infectious Bronchitis Virus Isolate Strain Jin-13 in SPF Chickens

作　　者：李欢[1] 杨霞[1] 赵军[1] 王忠田[2] 陈陆[1] 王新卫[1] 常洪涛[1] 李永涛[1] 刘红英[1] 王川庆[1] 

机构地区：[1]河南农业大学禽病研究所,郑州450002 [2]中国兽药监察所,北京100081

出　　处：《病毒学报》2014年第4期353-358,共6页Chinese Journal of Virology

基　　金：农业科技成果转化资金项目(2011GB2D000007)

摘　　要：本研究旨在掌握禽传染性支气管炎病毒（Infectious bronchitis virus,IBV）感染SPF鸡后在体内的动态分布。针对IBV流行毒Jin-13株N基因设计并合成一对引物,建立了检测IBV Sybr green I荧光定量PCR方法。人工感染90日龄SPF鸡1、4、7、10、14、21、28和35d后检测心脏、肝脏、脾脏、肺脏、气管、肾脏和十二指肠等内脏器官病毒载量,以研究体内病毒复制动态及分布情况。该方法在7.77×108~100拷贝/μL范围内具有良好的线性关系,可检测到初始模板中7.7拷贝/μL的病毒核酸,比常规RT-PCR方法敏感100倍。结果表明鸡群从感染后第4日开始发病,各组织器官内IBV病毒含量逐渐升高,至第7日肾脏病毒含量最高达到7.13×104拷贝/μL,其余依次为气管、肺脏、脾脏、心脏、肝脏和十二指肠。攻毒10日后各组织内含量均逐渐降低。肝脏于攻毒后21日时已检测不到IBV。于攻毒后第28日肾脏、气管和肺脏仍能检测到IBV。心脏可持续带毒35d。本研究从组织嗜性和动态分布方面为解释了IBV Jin-13株感染后的持续排毒及其严重的致肾损伤现象。This study aimed to understand the dynamic distribution of infectious bronchitis virus（IBV）Jin-13strain in SPF chickens.Ninety-day-old SPF chickens were inoculated with Jin-13,a virulent strain,and dissected at day 1,4,7,10,14,21,28or 35post-inoculation（dpi）.Samples of heart,liver,spleen,lung,trachea,kidney and duodenum were collected and the N gene was detected by Sybr Green I real-time quantitative RT-PCR assays.The established method had a good linear correlation from 7.77×108 to 100copies/μL.SPF chickens developed typical clinical signs of IBV at the 4th dpi,and the IBV viral concentration of tissues and organs gradually increased with a peak of up to 7.13×104 copies/μL.The viral concentration of most organs decreased by the 10th dpi,but those of the kidney,trachea and lung remained positive for IBV at 28dpi and the heart was still positive for IBV at＆gt; 35dpi.The results of this study,showed that the Jin-13strain can cause prolonged virus excertion in chickens with severe renal damage.

关 键 词：传染性支气管炎病毒 Jin-13分离株 SPF鸡 荧光定量PCR 动态分布 

分 类 号：S852.65[农业科学—基础兽医学]