兰州地区腹泻患儿中人博卡病毒1~4型流行病学研究  

Etiological Study of Human Bocavirus l-4 in Children with Acute Diarrhea in Lanzhou,China

作　　者：向静瑶[1] 李丹地 马鑫[3] 郭延青 段招军 李宇宁[1] 

机构地区：[1]兰州大学第一医院儿科,甘肃730000 [2]中国疾病控制预防控制中心病毒病预防控制所病毒性腹泻室,北京102206 [3]宁夏医科大学检验学院,银川750004

出　　处：《病毒学报》2014年第4期402-407,共6页Chinese Journal of Virology

摘　　要：了解兰州地区病毒性腹泻患儿中人博卡病毒1~4型（HBoV1~4型）的流行情况及临床特点,并探讨HBoV与急性胃肠炎的疾病相关性。收集兰州大学第一医院2012年7月至2013年6月5岁以下腹泻患儿的粪便标本331份,采用PCR方法检测人博卡病毒,同时检测常见的肠道病毒。331份标本中共检出博卡阳性标本49例（14.80%）,其中HBoV1~4型分别检测出26例、15例、7例和1例。分析其流行规律发现HBoV相关的腹泻全年散发,无明显季节分布。HBoV感染的患儿年龄为11.04±6.92月龄,高发年龄是7~12月龄。2岁以下患儿占HBoV阳性患儿总数的93.88%。HBoV与其他病毒混合感染率为71.3%,以混合轮状病毒为主。HBoV感染对腹泻患儿的发热和呕吐发生率无明显影响。检测出一例罕见的HBoV4病毒LZFB086,与泰国（序列号JQ267789）参考株的同源性为99.0%。未检测出HBoV2B型。从研究结果得出我国兰州地区人博卡病毒以HBoVl为主,在我国首次发现HBoV4病毒。HBoV1~4与其他病毒的混合感染率高,主要是混合轮状病毒。HBoV可能不是导致急性胃肠炎的致病病原。This study aimed to study the epidemiological and clinical characteristics of human bocavirus 1-4（HBoV1-4）in children with acute diarrhea in Lanzhou and to investigate the association between HBoV and acute gastroenteritis.A total of 331stool samples were collected from children aged under 5years with acute diarrhea at the Department of Pediatrics,the First Hospital,Lanzhou University,between July 2012and June 2013.Nested PCR was used to screen for HBoV and a general PCR was employed to screen other common diarrhea viruses.We found human bocavirus 1,2,3and 4in 26,15,7and 1cases,respectively.There was no specific seasonal distribution of HBoV,with infections occurring throughout the year.HBoV was mostly found in children aged between 7and 12months,with a mean age of 11.04months（±6.92months）,and 93.88%of affected children were aged under 2years.Overall,71.3%of mixed infections were mixed and the majority of other infections were caused by rotavirus.There was no statistical difference in the incidence of fever and vomiting associated with HBoV infection.A rare virus strain,HBoV4（LZFB086）,was identified,which showed highest levels of nucleotide sequence identity（99.0%）with a single Thai HBoV strain（JQ267789）.No case of HBoV2Bwas found.In conclusion,HBoV1was a major etiological pathogen of HBoV in pediatric cases in Lanzhou.HBoV4was detected in feces for the first time in China.The rate of mixed infections was high and rotavirus was dominant.The data presented suggests that HBoV is not a major causative agent of gastroenteritis.

关 键 词：腹泻 人博卡病毒 兰州 

分 类 号：R373.9[医药卫生—病原生物学]