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作 者:张乃菊[1,2] 余美玲[1,2] 张鑫宇[2] 祝晓光[2]
机构地区:[1]蚌埠医学院第一附属医院,蚌埠233000 [2]蚌埠医学院药学系
出 处:《中国药事》2014年第7期746-749,共4页Chinese Pharmaceutical Affairs
基 金:安徽省教育厅自然科学重点研究资助项目(编号NO2005KJO47ZD)
摘 要:目的体外观察赖氨匹林对转染并稳定表达连接蛋白32/26(Cx32/Cx26)的Hela细胞缝隙连接通讯(GJIC)功能和Cx32/Cx26蛋白表达水平的影响。方法不同浓度(0、1、5、10mmol·L-1)赖氨匹林作用于人宫颈癌Hela细胞24h后,以划痕标记/染料示踪技术(SL/DT)检测赖氨匹林作用Hela细胞48h后荧光黄在细胞之间传递的距离来评价其对缝隙连接(GJ)功能的影响;Western blot方法检测赖氨匹林对Cx32/Cx26蛋白表达的影响。结果 SL/DT检测显示赖氨匹林具有上调GJ功能的作用;Western blot显示赖氨匹林增加Cx32/Cx26蛋白表达水平。结论赖氨匹林抑制转染并稳定表达Cx32/Cx26的Hela细胞的增殖、上调GJ功能且这种作用可能与其增加Cx32/Cx26蛋白表达水平有关。Objective To investigate the effect of aspisol on gap junction (GJ) function in Hela cells transfected with connexin 32 and connexin 26 (Cx32/Cx26) plasmid and expression of Cx32/Cx26 protein. Methods Hela cells were incubated with aspisol at various concentrations (0, 1, 5, 10 mmol ·L^-1) for 24 h. The GJ function of Hela cells treated with aspisol for 48 hours was detected by scrape-loading dye transfer (SL/DT), and the effect on GJ function was evaluated by the transmission distance of lucifer yellow between cells. Western blotting was performed to detect the expression level of Cx32/Cx26 increased by aspisol. Results SL/DT showed that aspisol upregulated the function of GJ of Hela cells. Western blotting showed that aspisol increased the Cx32/Cx26 protein expression. Conclusion Aspisol can inhibit proliferation of Hela cells transfected with connexin 32 and connexin 26 plasmid which stably expressed Cx32/Cx26 and upregulate GJ function. These effects are possibly associated with the increase of Cx32/Cx26 expression indoced by aspisol.
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