汉黄芩素对小胶质细胞活化和迁移的影响  

Effects of wogonin on activation and migration of microglia

作　　者：陈正爱[1] 朴花子[1] 

机构地区：[1]延边大学医学院药理学教研室,吉林延吉133002

出　　处：《延边大学医学学报》2014年第2期98-101,共4页Journal of Medical Science Yanbian University

摘　　要：[目的]观察汉黄芩素对小胶质细胞活化和迁移的影响.[方法]建立小胶质细胞体外培养模型,给予脂多糖(LPS,100μg/L)及不同浓度的汉黄芩素(10,20,30μmol/L)后再培养12 h,采用ELISA法测定分泌到细胞外液中的单核细胞趋化因子(MCP-1)和激活调节正常T细胞表达和分泌因子(RANTES)的含量.采用Transwell细胞培养室检测小胶质细胞的迁移,观察汉黄芩素对MCP-1诱导的小胶质细胞迁移的影响.[结果]LPS高度激活小胶质细胞并使MCP-1和RANTES分泌增加,汉黄芩素明显抑制MCP-1和RANTES的分泌(P<0.05).MCP-1诱导小胶质细胞增加迁移率,而汉黄芩素显著抑制MCP-1诱导的小胶质细胞迁移(P<0.01).[结论]汉黄芩素可抑制小胶质细胞的迁移率,其机制可能与抑制趋化因子MCP-1和RANTES的分泌有关.OBJECTIVE To study the effects of wogonin on activation and migration of microglia.METHODS The microglial cells were incubated with LPS（100μg/L）and different concentrations of wogonin（10,20,30μmol/L）for 12 h,and the contents of monocyte chemotactic protein-1（MCP-1）and regulated upon activation normal T cell expressed and secreted（RANTES）were measured by ELISA.The migration of microglia was tested with a transwell migration assay to observe the effect of wogonin on MCP-1 induced migration of microglia.RESULTS The microglia increased markedly the secretion of MCP-1and RANTES in response to LPS,and the wogonin inhibited significantly the secretion of MCP-1and RANTES in microglial cells.MCP-1induced the migration of microglia cells,and the wogonin suppressed markedly the MCP-1induced migration of microglia cells（P〈0.01）.CONCLUSIONThe wogonin is able to attenuate the migration of microglia through autocrine suppression of chemokines production,such as MCP-1and RANTES.

关 键 词：小胶质细胞 汉黄芩素 单核细胞趋化因子 

分 类 号：R285.5[医药卫生—中药学]