Regulation of shear stress on rolling behaviors of HL-60 cells on P-selectin  

作　　者：LING YingChen FANG Ying YANG XiaoFang LI QuHuan LIN QinYong WU JianHua 

机构地区：[1]School of Life Sciences, Sun Yat-Sen University [2]School of Bioscience and Bioengineering, South China University of Technology [3]Institute of Biomechanics, South China University of Technology

出　　处：《Science China(Physics,Mechanics & Astronomy)》2014年第10期1998-2006,共9页中国科学：物理学、力学、天文学（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.11272125,11072080,31170887 and 31200705);Guangdong Natural Science Foundation(Grant No.S2011010005451);Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20110172110030)

摘　　要：Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1 （PSGL-1）, and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm：. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing frac- tional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mecha- nism for most cell rolling events through P-selectin.Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1(PSGL-1), and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm2. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing fractional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mechanism for most cell rolling events through P-selectin.

关 键 词：P-SELECTIN P-selectin glycoprotein ligand-1 cell adhesion shear stress 

分 类 号：R346[医药卫生—基础医学]