端粒与心力衰竭心肌纤维化的相关性研究  

Correlation between telomeres and myocardial fibrosis in heart failure

作　　者：魏玲[1] 付莉[1] 杨晓华[1] 高红[1] 李丽娟[1] 刘茜[1] 马玲[1] 贾政[1] 陈韦强 甘世保[1] 史战国[1] 熊云兰[1] 朱姿英[1] 

机构地区：[1]成都军区昆明总医院心内科,昆明650032

出　　处：《临床心血管病杂志》2014年第7期640-644,共5页Journal of Clinical Cardiology

摘　　要：目的:探讨端粒与心力衰竭(心衰)发病的关系,以及端粒在心脏重构、心肌纤维化中的作用。方法:将住院患者分为心衰组60例,心功能正常组60例。采用实时荧光定量(RT-PCR)方法检测外周血白细胞中端粒长度;应用ELISA方法检测上述60例患者血液标本血清中PⅠCP,PⅢNP的浓度,并与端粒长度、心功能分级及年龄等因素进行相关性分析;部分患者行心肌组织的HE染色。结果:①心衰组外周血白细胞端粒长度明显缩短;端粒长度与心功能分级呈负相关;②心衰组患者血清PⅠCP、PⅢNP含量明显增加,与心功能分级呈正相关;③心衰患者端粒长度与血清中PⅢNP呈独立的、显著的负相关;④病理切片HE染色提示,心衰患者病理切片中可见大片状胶原纤维沉积,心功能正常组少量胶原纤维沉积。结论:端粒的长度与心衰的发生发展,及心肌纤维化机制密切相关。Objective：This study is to investigate the relationship between telomeres and heart failure and the role of telomeres in heart remodeling and myocardial fibrosis to further definite the role of aging in the onset and progress of heart failure.Method：A total of 120 patients were divided into two groups,heart failure group and normal cardiac function group,each with 60 participants.We used real time fluorescence quantitative（FQ-PCR）to detect telomere length of the peripheral white blood cells and ELISA to test the serum concentration of PⅠ CP,PⅢNP.Myocardial fibrosis was detected by HE dyeing.Result：①In HF group,the telomere length of peripheral blood leukocyte significantly shortened;And telomere length was demonstrated to be negative correlated with cardiac function classification.②The concentration of serum PⅠ CP,PⅢNP in the HF group increased significantly;The content and age were positively related to cardiac function classification.③Peripheral blood leukocyte telomere length in the HF group were independent,significantly negative correlated to serum PⅢ NP.④By performing pathological HE dyeing of the two groups,it was demonstrated that there was a large patch of collagen deposition in the heart failure patients while a few collagen deposition in the nomal heart funcion group.Conclusion：The length of telomere is greatlycorrelated with myocardial fibrosis and the progression of HF.

关 键 词：心力衰竭 端粒 心脏结构重构 心肌纤维化 

分 类 号：R541.6[医药卫生—心血管疾病]