气相色谱-质谱联用法检测人尿中2-氨基-N-乙基-1-苯基丁烷  

Detection of 2-amino-N-ethyl-1-phenylbutane in Human Urine by Gas Chromatography-mass Spectrometry

作　　者：邓静[1] 王杉[1] 王小兵[1] 徐友宣[1] 

机构地区：[1]国家体育总局反兴奋剂中心,北京100029

出　　处：《中国运动医学杂志》2014年第5期453-456,共4页Chinese Journal of Sports Medicine

摘　　要：采用气相色谱-质谱联用检测技术,建立人尿中2-氨基-N-乙基-1-苯基丁烷兴奋剂的检测方法。样品采用碱性条件下液-液分配提取,以Hp-5（17 m×0.2 mm×0.33μm）色谱柱分离,质谱检测,Scan方式采集。按照世界反兴奋剂机构（WADA）认可实验室方法验证程序进行验证,该方法的检测限为20 ng/mL,回收率为70%~108%（n=6）,精密度RSD为2.2%,满足常规检测要求。The method was established for detecting 2-amino-N-ethyl-1-phenylbutane in human urine.The urine sample was prepared by liquid-liquid extraction in alkaline solution and separated with Hp-5（17 m×0.2 mm×0.33 μm）column and detected by gas chromatography-mass spectrometry in SIM mode.The method was evaluated according to the requirements of World Anti-Doping Agency.The detection limit for target analyte was 20ng/mL.The recovery and precision were 70-108%（n = 6）and2.2%,respectively.It was concluded that the method was suitable for routine doping control.

关 键 词：气相色谱-质谱联用仪 刺激剂 兴奋剂检测 

分 类 号：R87[医药卫生—运动医学]