不规则抗体筛选在新生儿溶血病中的临床诊疗  

Application of irregular antibodies detection in clinical diagnosis of hemolytic disease of newborn

作　　者：张樱子[1] 赵玉河[1] 高冀辉[1] 张晨光[2] 

机构地区：[1]新乡医学院第一附属医院输血科,河南新乡4531002 [2]新乡医学院检验系

出　　处：《临床血液学杂志（输血与检验）》2014年第4期656-657,661,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：河南省科技厅重点项目(No:132102310163)

摘　　要：目的:探讨不规则抗体筛选在新生儿溶血病中的临床诊疗意义。方法:随机选取662例新生儿溶血病患儿,采用微柱凝胶免疫检测技术对其血清进行不规则抗体筛选。结果:共计检测有6例不规则抗体阳性结果。特异性抗体分别为抗-E 3例、抗-c 1例、抗-Ce 1例、抗-D 1例,6例患儿均为重症新生儿溶血病,经选用与患儿配合型血液换血治疗结合光疗和药物治疗,2周后痊愈出院。结论:不规则抗体筛选在新生儿溶血病诊断中意义重大,在协助诊断新生儿免疫性溶血性疾病方面优点尤其突出。早期适时对不规则抗体进行筛选,可以使重症新生儿溶血病患儿更早找到合适血源,减少输血等待时间,对保证新生儿输血安全十分重要。Objective：To investigate the clinical diagnosis significance of irregular antibodies diction in hemolytic disease of newborn. Method：662 samples of blood recipients were screened and identified for irregular antibodies by adopting the microcolumn gel immunoassay technique supported by Changchun Boxun Biotechnology LTD. Resuit：The irregular antibodies were detected in 6 cases by microcolumn gel immunoassay technique, and there were 3 anti-E, 1 anti-c, 1 anti-Ce and 1 anti-D. All of the six newborn children were suffering severe haemolytic disease. By selecting the fit type blood and taking blood exchange transfusion therapy combined with phototherapy and drug treatment, all of them were completely cured two weeks later. Conclusion：It is extremely significant to apply irreg- ular antibodies screening to the hemolytic disease diagnosis for the newborns, so as to assist in the diagnosis of neonatal immune hemolytic disease. Timely screening of irregular antibodies could help severe hemolytic disease of newborn children find a fit blood type as soon as possible, and consequently help reduce the waiting time for blood transfusion, which is extremely important for the neonatal transfusion safety.

关 键 词：不规则抗体 新生儿溶血 输血安全 微柱凝集法 抗人球蛋白实验 

分 类 号：R457.1[医药卫生—治疗学]