YY1在胃癌组织中的表达及其对SGC-7901细胞株增殖和凋亡的影响  

Expression of YY1 in gastric cancer and its effect on the proliferation and apoptosis of gastric cancer cell line SGC-7901

作　　者：谢坤领 张静静[1] 朱毅[1] 徐泽宽[1] 

机构地区：[1]南京医科大学第一附属医院普外科,江苏南京210029 [2]亳州市人民医院普外科,安徽亳州236800

出　　处：《南京医科大学学报（自然科学版）》2014年第8期1010-1014,1024,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81361120398;81072031;81272712)

摘　　要：目的:检测YY1在胃癌组织中的表达情况,探讨YY1对人胃癌细胞株SGC-7901增殖、凋亡的影响以及可能的机制。方法:利用定量PCR和免疫组织化学分别检测标本中YY1的mRNA和蛋白表达水平。利用慢病毒表达载体系统在胃癌细胞株SGC-7901中上调YY1,运用CCK-8、流式细胞术、平板克隆实验检测YY1对细胞的增殖、凋亡以及克隆形成能力的影响。结果:胃癌组织中YY1的mRNA和蛋白表达水平明显低于癌旁组织。与空载对照组(SGC-7901-Empty Vector,SGC-7901-EV)和与正常对照组(SGC-7901)相比,过表达YY1组细胞(SGC-7901-YY1)的增殖和平板克隆形成能力明显减弱,细胞凋亡增加。结论:YY1与胃癌的发生发展有一定的关系,可能起到抑癌基因的作用,YY1基因可能成为胃癌靶向治疗的新的潜在靶点。Objective：To investigate the expression level of YY1 in gastric cancer,and then detect the effect of YY1 on the proliferation and apoptosis in SGC-7901 cell lines and its possible mechanism.Methods：mRNA and protein expressions of YY1 in cancer and paired adjacent tissues were detected by real-time PCR and immunohistochemistry,respectively.YY1 was up-regulated by a lentiviral vector expression system in gastric cancer cell line SGC-790.CCK-8 was performed to detect cell proliferation,and colony formation assay was performed to evaluate cell growth.Apoptosis was detected by flow cytometry.Results：The expression of YY1 in paired adjacent tissues had a higher level than that in tumor tissues.Compared to the empty vector group（SGC-7901-Empty Vector /SGC-7901-EV） and the normal control group（SGC-7901）,the cell proliferation and clonogenicity were significantly decreased as well as cell apoptosis was increased in the over-expressed YY1 group（SGC-7901-YY1）.Conclusion：YY1 may participate in the development of gastric cancer.YY1 can play a role as a cancer suppressor gene in the development of gastric cancer,which suggests that YY1 may be a new therapeutic target for gastric cancer.

关 键 词：胃癌 YY1 细胞增殖 细胞凋亡 

分 类 号：Q257[生物学—细胞生物学]