新城疫病毒D90株对乳腺癌MCF-7细胞作用的实验研究  

Anticancer effects of NDV-D90 on breast cancer cells MCF-7

作　　者：张增岭[1] 蔡永东[2] 宋纯[3] 胡昇庠[1] 

机构地区：[1]南京医科大学附属南京医院浦口分院,南京市浦口区中心医院普外科,江苏南京211800 [2]南京医科大学附属南京医院普外科,江苏南京210006 [3]哈尔滨医科大学附属第一临床医学院乳甲外科,黑龙江哈尔滨150001

出　　处：《南京医科大学学报（自然科学版）》2014年第8期1066-1071,共6页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:国内外研究表明,新城疫病毒在体内外对多种肿瘤具有明显抑制作用。本研究旨在探讨新城疫病毒D90株体外对乳腺癌MCF-7细胞的作用及可能机制。方法:CCK-8法检测MCF-7细胞增殖抑制;光学显微镜及DAPI染色观察D90株对MCF-7细胞形态学影响;流式细胞术检测MCF-7细胞凋亡率;Western blot检测MCF-7细胞凋亡相关蛋白的表达情况。结果:D90株对MCF-7细胞具有显著抑制作用并呈浓度和时间依赖性;D90株能够诱导MCF-7细胞凋亡并调节凋亡相关蛋白的表达,包括上调Bax蛋白、下调Bcl-2蛋白,并促进Caspase-3和Caspase-9的裂解活化。结论:D90株能够调节凋亡相关蛋白的表达,并促进Caspase-3和Caspase-9的裂解活化,通过线粒体途径诱导MCF-7细胞凋亡并呈浓度和时间依赖性。Objective：Studies showed that Newcastle disease virus（NDV） had significant inhibitory action on many tumors in vitro and in vivo.This experiment was designed to investigate the effects and possible mechanism of NDV-D90 strain on breast cancer MCF-7 cells in vitro.Methods：Growth inhibition of MCF-7 cells was detected by CCK-8 method.The morphological changes of MCF-7 cells caused by D90 strain were observed by optical microscope and DAPI staining.MCF-7 cell apoptosis rate was detected by flow cytometry.The expression of apoptosis related proteins of MCF-7 cells was detected by Western blot.Results：NDV-D90 strains had inhibitory effect on MCF-7 cells in a concentration and time dependent manner;D90 strain induced apoptosis of MCF-7 cells and regulated the expression of apoptosis related proteins,including up-regulating Bax expression,down-regulating Bcl-2 protein,and promote the cleavage and activation of Caspase-3 and Caspase-9.Conclusion：D90 strain could regulate the expression of apoptosis related protein,promote the cleavage and activation of Caspase-3 and Caspase-9.It could induce apoptosis of MCF-7 cells through the mitochondrial pathway in a concentration and time dependent manner.

关 键 词：新城疫病毒 乳腺癌 MCF-7细胞 凋亡 调亡蛋白 

分 类 号：Q255[生物学—细胞生物学]