VDAC2基因多态性与原发性不育症男性精液质量的相关性分析  

Association of the VDAC2 gene polymorphism with semen quality in Han-Chinese population with idiopathic male infertility

作　　者：章轶[1,2] 赵丹[1,3] 徐璞[4] 吴炜[1,5] 王增军[1,2] 王心如[1,5] 

机构地区：[1]南京医科大学生殖医学国家重点实验室,江苏南京210029 [2]南京医科大学第一附属医院泌尿外科,江苏南京210029 [3]南京医科大学附属南京妇幼保健院泌尿外科,江苏南京210029 [4]南京医科大学附属南京妇幼保健院妇科,江苏南京210029 [5]南京医科大学现代毒理学教育部重点实验室,卫生毒理学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2014年第8期1087-1091,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(30872575,30973199)

摘　　要：目的:探讨VDAC2基因多态性与原发性不育症男性精液质量的相关性。方法:收集523例原发性不育男性患者的精液和血液,分别应用计算机辅助精液分析系统(CASA)行精液质量分析和应用TaqMan SNP基因分型技术对VDAC2基因位点rs2804535、rs7896741、rs11001334和rs1259503行基因分型,分析VDAC2基因多态性与精液质量的相关性。结果:没有发现rs2804535和rs7896741各基因型与精液质量相关,但是rs11001334中,TT基因型与CC基因型比较,精子浓度显著降低(P=0.045)。CT基因型和CT+TT基因型分别与CC基因型比较,精子活力显著增加(P=0.026,P=0.037);rs1259503的GC+CC基因型与GG基因型比较,精液量显著降低(P=0.039)。结论:VDAC2基因多态性与原发性不育症男性精液质量相关。Objective：To analyze the possible association between VDAC2 gene polymorphism and sperm quality of idiopathic male infertility.Methods：A genetic typing of VDAC2 gene locuses（rs2804535,rs7896741,rs11001334 and rs1259503） by TaqMan SNP genotyping technique and a semen analysis by computer-assisted semen analysis（CASA） were respectively performed in 523 HanChinese males with idiopathic infertility in order to analyze the correlation between gene polymorphism and sperm quality.Results：No significant association between genotypes and semen quality was found at locuses rs2804535 and rs7896741.However,at rs11001334,it was found that the TT genotype showed a significant lower sperm concentration compared with the CC genotype（P =0.045）.Moreover,compare with CC genotype,the sperm motility of CT genotype had a significant increase（P = 0.026）;meanwhile,the same trend was found in the combination of CT and TT genotypes（P = 0.037）.However,at rs1259503,the combination of GC and CC genotypes had a remarkable reduction of semen volume than GG genotype（P = 0.039）.Conclusion：These findings indicate that the gene polymorphism in the VDAC2 gene may be associated with semen quality.

关 键 词：VDAC 基因多态性 男性不育 精液 

分 类 号：Q786[生物学—分子生物学]